miR-130b和RUNX3mRNA在胃癌组织中的表达及其临床意义

Expression of RUNX3 and miR-130b in gastric carcinoma and clinical significance

目的研究miR-130b和RUNX3mRNA在胃癌组织中的表达，并评价miR-130b对抑癌基因RUNX3表达的影响。方法用荧光定量PCR技术检测40对胃癌组织及其对应的癌旁正常胃黏膜组织中miR-130b和RUNX3mRNA的表达量；用免疫组织化学SP法检测RUNX3蛋白的表达。结果胃癌组织中miR-130b的表达显著高于对应的癌旁组织（2．18±3．75）比（2．59±3．45），P〈0．05；而胃癌组织中RUNX3mRNA的表达显著低于对应的癌旁组织（8．76±2．82）比（7．58±2．87），P〈0．05。miR-130b和RUNX3mRNA的表达与胃癌的淋巴结转移、临床分期有关（P〈0．05），而与患者的年龄、性别、肿瘤大小、远处转移、有无浸润周边器官组织无关（均P〉0．05）。miR-130b的表达与RUNX3蛋白的核表达、细胞核＋细胞质的表达存在负相关（P〈0．05），而与RUNX3mRNA、RUNX3蛋白的细胞质表达无相关性（P〉0．05）。结论miR-130b和RUNX3mRNA的异常表达与胃癌预后有关；miR-130b可能通过减少RUNX3蛋白的表达水平导致肿瘤的发生。

Objective To explore the expression of miR-130b and RUNX3mRNA in human gastric carcinoma and the clinical significance. Methods The expression of miR-130b and RUNX3mRNA were detected by RT-PCR in 40 cases of gastric carcinoma and corresponding normal mucosa tissue. The expression of RUNX3 protein was determined by immunohistochemistry SP method. Results The expression of miR-130b was significantly up-regulated in gastric carcinoma than that in the adjacent normal gastric mucosa tissues （2. 18 ±3.75） vs. （2. 59 ±3.45）, P 〈 0.05 ; The expression of RUNX3mRNA in gastric carcinoma tissues was significantly lower than that in adjacent normal gastric mucosa tissues（8. 76 ± 2. 82） vs. （ 7. 58 ± 2. 87 ）, P 〈 0.05. The expression of miR-130b and RUNX3mRNA were positively correlated with lymph node metastasis and clinical stage （ P 〈 0.05 ） ; No significant association was found between the expression and age, gender, tumor size, distant metastasis and depth of tumor invasion （P 〉 0. 05 ）. The expression of miR-130b was negatively correlated with RUNX3 protein expression in nuclei and cytoplasm （ P 〈 0. 05 ）. Conclusions Abnormal expression of miR-130b and RUNX3mRNA correlates with prognosis of gastric carcinoma ; Decreased RUNX3 protein expression may contribute to tumourigenesis.