摘要
目的探讨黄芪甲苷(astragaloside Ⅳ)预处理对小鼠肝脏缺血再灌注损伤的保护作用及其机制。方法将60只雄性C57BL/6小鼠分为4组,15只/组,A组:假手术对照组,B组:假手术黄芪甲苷组,C组:实验对照组,D组:黄芪甲苷实验组。黄芪甲苷组每只腹腔注射黄芪甲苷24mg·kg^-1·d^-1,对照组每天腹腔注射等体积无菌生理盐水,共1周。建立小鼠肝脏部分缺血再灌注损伤模型,复流24h后采集标本,测定血清中转氨酶ALT和AST水平,光镜下观察H&E染色肝组织病理学变化,酶联免疫吸附试验(ELISA)检测血清中IL-1β,IL-6,TNF-α的含量,采用Western blot技术检测小鼠肝脏组织中核因子-κB(NF-κB)的表达。结果黄芪甲苷实验组ALT及AST较实验对照组明显降低[AST:C组(4290±292)U/L vs.D组(2373±416)U/L,t=0.844;ALT:C组(4146±500)U/L vs.D组(2318±289)U/L,t=7.08,均P〈0.05],组织学损伤也明显减轻;ELISA结果显示,黄芪甲苷预处理能明显降低外周IL-1β,IL-6,TNF-α的含量(IL-1β:t=10.04;IL-6:t=6.281;TNF-α:t=6.817;均P〈0.05)。黄芪甲苷实验组肝脏组织中NF—κB表达量明显低于实验对照组。结论黄芪甲苷预处理对小鼠肝脏缺血再灌注损伤有明显的保护作用。
Objective To investigate the protective effect of astragaloside Ⅳ pretreatment against liver ischemia/reperfusion (I/R) injury in mice. Methods Sixty male C57BL/6 mice were randomized into four groups (15 mice in each group): group A: sham surgery with saline injection, group B: sham surgery with astragaloside Ⅳ injection, group C: I/R group with saline injection, group D: I/R and astragaloside Ⅳ injection. Mice were pretreated by daily intraperitoneal injection of saline or astragaloside IV (24 mg · kg^-1 · d^-1 ) for one week. The mouse partial liver model of I/R injury was established, and samples were collected at the 24 h after the I/R injury. Serum ALT and AST levels were determined, the histologic changes were observed by H&E staining under the light microscopy, whereas the nuclear factor (NF)-κB was assessed with Western blotting. Serum IL-1β, IL-6, and TNF-α levels were measured by enzyme-linked immunosorbent assay (ELISA). Results Serum ALT and AST levels significantly decreased and the histological damage was significantly alleviated in astragaloside Ⅳ treated I/R group as compared with saline I/R group [AST: C: (4290 ±292) U/L vs. D: (2373 ±416) U/L t =0. 844;ALT: C: (4146 ±500) U/L vs. D:(2318 ±289) U/L t =7.08 P 〈0.05]. In comparison with group 3, astragaloside Ⅳ reduced NF-κB nuclear expression. ELISA showed astragaloside Ⅳ significantly inhibit the levels of IL-1β, IL-6, and TNF-α in the serum ( IL-1β: t = 10. 04; IL-6: t = 6. 281; TNF-α: t = 6. 817; P 〈 O. 05 ). Conclusions Pretreatment with astragaloside Ⅳ effectively protect against liver ischemia/reperfusion injury in mice.
出处
《中华普通外科杂志》
CSCD
北大核心
2012年第9期747-750,共4页
Chinese Journal of General Surgery
基金
江苏省卫生厅面上科研课题基金资助项目(H200870)
常州市卫生局重大招标基金资助项目(ZD200811)
关键词
黄芪
再灌注损伤
NF-ΚB
Astragalus membranaceus
Repeffusion injury
NF-kappa B