乳腺癌中S100A7与AKT的表达及意义

Detection of S100A7 and AKT expression in breast cancer

目的探讨乳腺癌中S100A7和AKT在乳腺癌中的表达及其相关性。方法采用免疫组化PV非生物素两步法检测226例乳腺癌中S100A7与AKT的表达及与ER、PR、Her-2表达的关系。结果 S100A7蛋白在乳腺导管原位癌中阳性率增高(52.50%),AKT在乳腺浸润性导管癌中阳性率高(50.00%);S100A7和AKT在伴淋巴结转移的病例中阳性率高(P=0.0062,P=0.044 8);且Ⅲ期乳腺癌阳性率高于Ⅰ和Ⅱ期(P=0.025 8,P=0.040 3)。AKT表达和肿瘤大小相关,差异有显著性(P=0.031 0);S100A7和AKT蛋白在乳腺癌中的表达与ER、PR呈负相关,与Her-2呈正相关,两者之间呈正相关。结论探讨S100A7与AKT蛋白在乳腺癌中的表达具有重要意义,两者有可能成为乳腺癌治疗的新的靶点。

Purpose To understand the different expression of S100A7 and AKT in breast cancer and their correlations. Methods Immunohistochemical PV non-biotin two-step method was used to examine expression of SI00A7 and AKT in 226 cases of breast canc- er, and their correlations with chnicopathological features involving ER, PR, and Her-2 status in breast cancer. Results The expres- sion of S100A7 protein in ductal carcinoma in situ, was high （52. 50% ） , while the positive expression rate of AKT in breast invasive ductal carcinoma increased （50. 00% ） , the expression of both S100A7 and AKT was higher in cases with lymph node metastasis （P = 0. 006 2, P = 0. 044 8）, and the expression rate in stage Ⅲ was higher than that in stages I and Ⅱ （P = 0. 025 8, P = 0. 040 3 ）. The correlation of AKT expression with tumor size was significant （P = 0. 031 0）. The expression of S100A7 and AKT in breast cancer had negative correlation with ER and PR status, and positive correlation with Her-2 amplification. Conclusion Detection of S100A7 and AKT proteins in breast cancer cells plays an important role in the pathologic study, which likely provide new targets for breast cancer treatment.