徒手淋巴引流技术结合穴位点按对大鼠下肢创伤后肿胀的影响

Manual lymphatic drainage technology combined with acupoint-pressing for lower limbs swelling after trauma of rats

目的:探讨徒手淋巴引流技术(MLDT)结合血海、阳陵泉点按对下肢软组织创伤所致肿胀及TNF-α表达的影响。方法:Wistar大鼠80只随机分组为正常组(D组)、模型组(M组)、MLDT组(T组)、综合治疗组(Z组),D组8只,其余组各24只。M、T和Z组造成软组织创伤模型,T组运用MLDT作用于水肿组织,Z组在此基础上结合穴位点按。M、D组未做干预。分别于治疗后第1、3和7天采用容积法测量足爪肿胀程度,观察治疗前后肿胀体积差值变化;ELSIA法检测干预后各时间点大鼠血清TNF-α表达变化。结果:治疗后第1天TNF-α含量各组间比较差异无统计学意义,体积差值比较,T组<Z组<M组(P<0.05)。干预后第3及7天,体积差值及TNF-α水平评分均为Z组<T组<M组(P<0.05)。结论:徒手淋巴引流术结合穴位点按可明显降低TNF-α的表达水平,加速肿胀的消退。

Objective.. To explore the effectiveness of manual lymphatic drainage technology （MLDT） combined with Xuehai （SP10） and Yanglingquan （GB34） acupoint-pressing treating lower limbs swelling after soft tissue trau- ma. Methods： Eighty Wistar rats were randomly divided into normal, model, MLDT and combined treatment groups, with 8 rats in normal group, and 24 rats in the rest groups. Soft tissue trauma model was made in model, MLDT and combined treatment groups. Rats in MLDT group were treated by MLDT, and those in combined treat- ment group were treated hy MLDT comhined with acupoint-pressing treatment. Normal and model groups got not treatment. Volume-measuring method was applied to observe the foot swelling degree before and at 1st, 3rd, and 7th day after treatment. ELSIA was used to detect the expression of serum TNF-α Results： At the 1st day after treatment, there were significant differences in TNF-α among groups （P〈0.05）, the foot volume result revealed T group 〈Z group〈M group（P〈0.05）. At the 3rd and 7th day, the foot volume and TNF-α results revealed Z group〈T group〈M group （P〈0.05）. Conclusion： MLDT combined with acupoint-pressing can markedly decrease the expression of serum TNF-α and facilitate swelling vanishing.