多环芳烃污染对桑园土壤微生物结构及种群多样性的影响

Effect of PAHs pollution on microbial structure and population diversity of mulberry orchard soil

采用磷脂脂肪酸(PLFAs)分析法和变性梯度凝胶电泳(DGGE)考察了受多环芳烃(PAHs)污染的桑园3个区域的土壤微生物群落结构及种群多样性的变化.PLFAs分析结果表明,区域2中微生物PLFA总量最高,主要为细菌和真菌;聚类分析揭示,土壤中微生物的PLFAs主要分为3大类群;冗余分析表明,土壤PAHs污染程度对土壤微生物群落结构有一定的影响.DGGE指纹图谱分析结果显示,在PAHs污染较高区域,其电泳条带较多,且3个区域中Shannon指数和Simpson优势度差异达显著水平,区域2种群优势度较高;主成分分析表明,不同区域微生物的种群结构存在显著性差异.

Phospholipid fatty acids （PLFAs） analysis method and PCR-denaturing gradient gel eleetrophoresis （DGGE） technology were used to investigate microbial structure and population diversity of the soil sampled from the three areas of the mulberry orchard, which was contaminated by polycyclic aromatic hydrocarbons （PAHs）. The results of PLFAs analysis showed that total PLFA biomass of the microbes in area 2 was the highest, most of which were the bacteria and fungi. Clustering analysis of soil microbes revealed that the PLFAs were mainly divided into three groups. Redundancy analysis demonstrated that PAHs pollution degree had a certain effect on soil microbial community structure. DGGE fingerprint analysis showed that there were more electrophoresis bands in the areas with higher PAHs contamination, and the difference of Shannon index and Simpson dominance degree were significant among three areas. Population dominance degree of the soil in area 2 was the highest. Principal components analysis of DGGE profiles indicated that there were significant differences in population structure of the microbes in different area.