摘要
目的探讨柠檬酸钠(Citrate)与三氧化二砷(As2O3)联合应用对胃癌细胞的凋亡及其作用机制。方法用Citrate(终浓度为5mmol/L)和(或)不同浓度的As2O3(终浓度依次为5、10μmol/L)处理体外传代培养的胃癌细胞株SGC-7901和BGC-803。流式细胞仪检测细胞凋亡和细胞周期的变化;RT-PCR法观察凋亡相关基因Bcl-2、Mcl-1表达的变化。结果 Citrate与As2O3联合应用相对于单用Cit-rate或As2O3可显著提高诱导胃癌细胞凋亡率(P<0.05);与空白组相比,用药后G0/G1期细胞比例下降,G2/M期细胞比例上升,细胞周期阻滞于G2/M期,抗凋亡基因Bcl-2、Mcl-1表达明显下降。结论 Citrate与As2O3联合应用具有协同抗胃癌细胞的作用,其机制可能与细胞周期阻滞、增强诱导胃癌细胞凋亡以及调控凋亡相关基因的表达有关。
Objective To study the anti-tumor effects of citrate combined with arsenic trioxide on human gastric cancer cell in vitro and possible mechanism.Methods Citrate（final concentration of 5mmol/L） and（or）different concentrations of As2O3（final concentration was 5or 10μmol/L）treated in vitro cultured gastric cancer cell line SGC-7901and BGC-803.Flow cytometry was used to detect apoptosis and cell cycle changes.The apoptosis-related gene Bcl-2and Mcl-1expressions were identified by RT-PCR to observe.Results Citrate combining with As2O3had a stronger inhibition function on human gastric cancer cell line compared with citrate or As2O3（P〈0.05）alone.Compared with blank group,the cell cycle distribution was arrested at G2/M phase,and the proportion of G0/G1phase cell decreased and G2/M phase increased（P〈0.05）.The expression of bcl-2and mcl-1was significantly decreased（P〈0.05）.Conclusion Citrate combining with As2O3had the synergistic effects on gastric cancer cell.The mechanism might be related to cell cycle blocking and apoptosis-inducing effects through bcl-2and mcl-1genes.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第9期1064-1069,共6页
Cancer Research on Prevention and Treatment
基金
广西自然科学基金资助项目(0832118)
