Wnt-7a蛋白抑制单侧输尿管梗阻小鼠肾脏上皮细胞-间充质细胞转分化

Wnt-7a inhibites epithelial to mesenchymal transition in mice of unilateral ureteral obstruction model

目的 观察Wnt-7a蛋白对单侧输尿管梗阻（UUO）模型小鼠肾脏纤维化的拮抗作用.方法 18只雄性C57BL/6小鼠按随机数字法分为假手术组、UUO模型组和Wnt-7a蛋白治疗组,每组6只.于术后7d处死小鼠,留取梗阻侧肾组织,行Masson染色观察肾间质纤维化程度；免疫组化染色检测肾组织E-钙黏蛋白、α-平滑肌肌动蛋白（ α-SMA）、波形蛋白的表达；Western印迹法测定肾皮质中E-钙黏蛋白和 α-SMA的表达.结果 术后第7天,与假手术组相比,模型组体质量显著下降（P＜0.05）,肾组织间质纤维化相对面积显著增多（P＜0.05）,肾组织α-SMA及波形蛋白的表达显著增加（P＜0.05）,E-钙黏蛋白表达显著减少（P＜0.05）.与模型组相比,Wnt-7a蛋白治疗组体质量无明显改变（P＞0.05）,肾组织间质纤维化相对面积显著减少（P＜0.05）,α-SMA及波形蛋白的表达显著减少（P＜0.05）,E-钙黏蛋白表达显著增高（P＜0.05）.结论 Wnt-7a蛋白可以抑制肾脏上皮细胞,间充质细胞转分化的发生而减轻肾脏纤维化.

Objective To investigate the protective effects of Wnt-7a protein on renal interstitial fibrosis in mice of unilateral ureteral obstruction （UUO）model.Methods Eighteen male C57BL/6 mice were randomly divided into 3 groups：sham-operation group,the UUO model group and Wnt-7a treatment group.The body weight of mice was measured everyday.All the mice were sacrificed at thc seventh day after the operation.The left kidney was taken for histology evaluation and molecular biology assay.Masson＇s stain was performed as a main indicator of interstitial fibrosis.The expression of vimentin,α-smooth muscle actin,and E-adherin in renal tissue was detected by immunohistochemistry staining and the expression of α-smooth muscle actin and E-cadhe（nn） in renal tissue was detected by Western blotting.Results Compared with sham-operation group,body weight of the （,）odel group was significantly lower （P〈0.05）,and the relative area of interstitial fibrosis was significantly larger （P〈0.05）.Furthermore,the expression of vimentin and α-SMA was significantly up-regulated （P〈0.05）,and the expression of E-cadherin was significantly down-regulated （P〈0.05）.Compared with model group,all the above-mentioned abnormalities were restored to some extent and showed significant differences （P〈0.05） in Wnt-7a treatment group.Conclusion Wnt-7a protein can decrease the interstitial fibrosis by inhibiting epithelial to mesenchymal transition in UUO mice.