青枯病病菌对茄子相关防卫信号基因表达及活性氧含量的影响

Effect of Inoculation of Ralstonia solanacearum on Gene Expression in Defense Signaling Pathways and Accumulation of ROS in Eggplants

本文以茄子抗青枯病自交系和感病自交系为试材,在接种青枯病菌后,对调控抗性反应的不同信号传导基因进行表达特性分析。结果表明,在9个基因中,EDS1、PAD4、NPR1、SGT1和WRYK70等5个基因的表达均随着接种诱导时间延长而增加,而且在抗病自交系‘E-31’中的表达量要高于感病自交系‘E-32’;而JAR1、NDR1、EIN2和RAR1等基因的表达随着接种诱导时间的增加,表达水平变化不大。初步推断EDS1、PAD4、NPR1、SGT1和WRYK70可能与调节茄子抗青枯病抗性反应有关。同时茄子在接种后活性氧的含量均增加,但是感病植株的含量高于抗病植株。

The inbred lines ＇E-31＇ （resistant to bacterial wilt ） and ＇E-32＇ （susceptible to bacterial wilt） of eggplant （Solanaceae melongena L.） were induced after inoculation ofRalstonia solanacearum. The expressions of nine genes in signal pathway of disease resistance were identified in the PUS inbred lines of eggplant （Solanum melongena）, such as EDS1, PAD4, NPR1, SGT1, WRYK70, JAR1, NDR1, EIN2, RAR1. The results showed that the expression levels of EDS1, PAD4, NPR1, SGT1, WRYKTO increased with the addition of induction time, and their levels of expression were higher in resistant inbred line ＇E-31＇ than those in susceptible inbred line ＇E-32＇, but the levels of expression of others four genes didn＇t change in both resistance and susceptibility materials. The results deduced that five genes （EDS1, PAD4, NPR1, SGT1, WRYK70） might be related to regulation the resistance to bacterial wilt in eggplant. The contents of ROS were higher in ＇E-31 ＇and ＇E-32＇ after inoculation, and the content of ROS in susceptible plant was higher than that in resistant plant.