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氧化型低密度脂蛋白抗体对单个核细胞CD36 mRNA表达的影响 被引量：1

Impact of anti-OX-LDL antibodies on CD36 mRNA expression in monocytes
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摘要 目的:通过观察氧化型低密度脂蛋白(OX-LDL)抗体对单个核细胞CD36 mRNA表达的影响,探讨OX-LDL抗体影响泡沫细胞形成的可能机制。方法:U937细胞和新西兰兔外周血单个核细胞分别被分成4组:空白对照组(普通培养基孵育)、OX-LDL刺激组(培养基中添加50μg/L的兔抗人OX-LDL多克隆抗体)、抗体干预组(培养基中添加50μg/L的兔抗人OX-LDL多克隆抗体及100μg/L的纯化人OX-LDL)及单纯抗体组(培养基中添加100μg/L的纯化人OX-LDL),经培养24 h后,利用半定量RT-PCR技术分析CD36的mRNA表达水平。结果:无论在U937细胞或兔单个核细胞中,OX-LDL刺激组及抗体干预组CD36 mRNA的表达量均显著高于对照组,而经抗体干预后,CD36 mRNA表达量在U937细胞和在兔单个核细胞分别降低了约64.80%和35.18%,种属间差异有统计学意义。结论:抗OX-LDL抗体可以抑制单个核细胞CD36抗原的表达,从而抑制泡沫细胞形成过程中OX-LDL向细胞内的聚集。 AIM：To investigate the impact of antibodies to oxidized low-density lipoprotein（OX-LDL） on CD36 mRNA expression in monocytes and explore the mechanism underlying the impact on the formation of foam cells.METHODS： U937 cells and the monocytes of New Zealand rabbit were respectively cultured in vitro and divided into 4 groups： the control group（cultured in nutrient medium of RPMI1640）,the OX-LDL group（with additional OX-LDL of 50 μg/L in nutrient medium）,the OX-LDL＋Ab-OX-LDL group（with additional OX-LDL of 50 μg/L and Ab-OX-LDL of 100 μg/L in nutrient medium） and the Ab-OX-LDL group（with additional Ab-OX-LDL of 100 μg/L in nutrient medium）.After 24-hour culture,the expression of CD36 mRNA was detected by semi-quantitative RT-PCR.RESULTS： The expression of CD36 mRNA,either in the OX-LDL group or in the OX-LDL＋Ab-OX-LDL group,was higher than that in the control group.After intervened by Ab-OX-LDL,the expression was respectively down-regulated by 64.80% in U937 cells and 35.18% in the monocytes of rabbit,which was statistically significant between the two species.CONCLUSION： Antibodies to OX-LDL could negatively regulate the expression of CD36 mRNA in monocytes,and prevent monocyte intaking OX-LDL through the pathway of antigen CD36.
作者 王磊 宫剑滨 王璟 张启高 王立军
机构地区 南京军区南京总医院心脏内科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2012年第9期930-932,936,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 南京军区科技创新重点课题(09Z026)
关键词 动脉粥样硬化 CD36抗原 抗OX-LDL抗体 泡沫细胞 半定量逆转录聚合酶链反应 atherosclerosis； antigen CD36； anti-OX-LDL antibodies； foam cells； semi-quantitative RT-PCR
分类号 R392.11 [医药卫生—免疫学]
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参考文献14

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二级参考文献9

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共引文献2

同被引文献11

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细胞与分子免疫学杂志
2012年 第9期

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