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氧化型低密度脂蛋白抗体对单个核细胞CD36 mRNA表达的影响 被引量:1

Impact of anti-OX-LDL antibodies on CD36 mRNA expression in monocytes
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摘要 目的:通过观察氧化型低密度脂蛋白(OX-LDL)抗体对单个核细胞CD36 mRNA表达的影响,探讨OX-LDL抗体影响泡沫细胞形成的可能机制。方法:U937细胞和新西兰兔外周血单个核细胞分别被分成4组:空白对照组(普通培养基孵育)、OX-LDL刺激组(培养基中添加50μg/L的兔抗人OX-LDL多克隆抗体)、抗体干预组(培养基中添加50μg/L的兔抗人OX-LDL多克隆抗体及100μg/L的纯化人OX-LDL)及单纯抗体组(培养基中添加100μg/L的纯化人OX-LDL),经培养24 h后,利用半定量RT-PCR技术分析CD36的mRNA表达水平。结果:无论在U937细胞或兔单个核细胞中,OX-LDL刺激组及抗体干预组CD36 mRNA的表达量均显著高于对照组,而经抗体干预后,CD36 mRNA表达量在U937细胞和在兔单个核细胞分别降低了约64.80%和35.18%,种属间差异有统计学意义。结论:抗OX-LDL抗体可以抑制单个核细胞CD36抗原的表达,从而抑制泡沫细胞形成过程中OX-LDL向细胞内的聚集。 AIM:To investigate the impact of antibodies to oxidized low-density lipoprotein(OX-LDL) on CD36 mRNA expression in monocytes and explore the mechanism underlying the impact on the formation of foam cells.METHODS: U937 cells and the monocytes of New Zealand rabbit were respectively cultured in vitro and divided into 4 groups: the control group(cultured in nutrient medium of RPMI1640),the OX-LDL group(with additional OX-LDL of 50 μg/L in nutrient medium),the OX-LDL+Ab-OX-LDL group(with additional OX-LDL of 50 μg/L and Ab-OX-LDL of 100 μg/L in nutrient medium) and the Ab-OX-LDL group(with additional Ab-OX-LDL of 100 μg/L in nutrient medium).After 24-hour culture,the expression of CD36 mRNA was detected by semi-quantitative RT-PCR.RESULTS: The expression of CD36 mRNA,either in the OX-LDL group or in the OX-LDL+Ab-OX-LDL group,was higher than that in the control group.After intervened by Ab-OX-LDL,the expression was respectively down-regulated by 64.80% in U937 cells and 35.18% in the monocytes of rabbit,which was statistically significant between the two species.CONCLUSION: Antibodies to OX-LDL could negatively regulate the expression of CD36 mRNA in monocytes,and prevent monocyte intaking OX-LDL through the pathway of antigen CD36.
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2012年第9期930-932,936,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 南京军区科技创新重点课题(09Z026)
关键词 动脉粥样硬化 CD36抗原 抗OX-LDL抗体 泡沫细胞 半定量逆转录聚合酶链反应 atherosclerosis; antigen CD36; anti-OX-LDL antibodies; foam cells; semi-quantitative RT-PCR
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参考文献14

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