脂滴包被蛋白对鸡前脂肪细胞脂质蓄积的影响

Effect of perilipin 1 on chicken preadipocyte lipid accumulation

目的:探讨脂滴包被蛋白(perilipin 1)对鸡前脂肪细胞脂质蓄积的影响。方法:首先构建pcDNA3.1-perilipin 1基因真核表达载体,转染鸡原代前脂肪细胞,24h后添加油酸诱导前脂肪细胞分化。然后利用Western blot方法检测perilipin 1的表达;利用油红O提取比色法研究perilipin 1对鸡前脂肪细胞脂质蓄积的影响;利用Real-time RT-PCR方法分析其他与脂类代谢相关的重要基因(FAS、ACC、ATGL)的表达变化。结果:转染pcDNA3.1-perilipin 1基因真核表达载体能明显上调perilipin 1的表达。perilipin 1表达量升高后,鸡前脂肪细胞脂质蓄积能力增强,但其他与脂类代谢相关的重要基因(FAS、ACC、ATGL)的表达量没有发生明显的变化。结论:过表达perilipin 1促进鸡前脂肪细胞的脂质蓄积。

AIM：To explore the effect of perilipin 1 on chicken preadipocyte lipid accumulation.METHODS： Firstly,pcDNA3.1-perilipin 1 gene eukaryotic expression vector was constructed and transfected into primary cultured chicken preadipocytes.Twenty-four hours after transfection,chicken preadipocytes were cultured in complete medium with oleate,to induce preadipocyte differentiation.Then perilipin 1 was detected by Western blotting;the role of perilipin 1 in preadipocyte lipid accumulation was investigated by Oil Red O staining extraction assay;the expression levels of other genes（FAS,ACC and ATGL） related to the chicken adipocyte lipid metabolism were tested by real-time RT-PCR.RESULTS： pcDNA3.1-perilipin 1 gene eukaryotic expression vector upregulated the expression of perilipin 1 in the transfected chicken preadipocytes.Over-expression of perilipin 1 promoted chicken preadipocyte lipid accumulation,while there was no significant change in the expression levels of other chicken lipid metabolism related genes（FAS,ACC and ATGL）.CONCLUSION： Over-expression of perilipin 1 can promote chicken preadipocyte lipid accumulation.