房颤患者右心耳组织SIRT1表达以及与氧化应激的相关性

Expression of SIRT1 in right auricle tissues and the relationship with oxidative stress in patients with atrial fibrillation

目的:观察房颤患者右心耳组织SIRT1的表达及其与心房氧化应激的关系。方法:选择行心脏手术的风湿性心脏病患者共38例,按照是否合并房颤分为两组:持续性房颤组25例(AF组),其房颤持续时间超过6个月;窦性心律组13例(SR组)。在术中取其部分右心耳组织,采用免疫组化法检测右心耳组织SIRT1蛋白表达,同时测定右心耳组织匀浆中丙二醛(MDA)、总超氧化物歧化酶(SOD)活力和金属硫蛋白(MT)的含量。结果:免疫组化结果发现AF组患者SIRT1表达(45.8±4.03)%高于SR组(19.7±2.54)%,差异有统计学意义(P<0.05)。房颤组MDA为(7.24±1.05)nmol/mg,SOD为(1034.25±84.32)U/mg,MT为(7.21±1.46)μg/g,窦性心律组MDA为(3.01±0.47)nmol/mg,SOD为(723.63±65.23)U/mg,MT为(4.31±1.23)μg/g,两者相比有显著性差异(P<0.05)。房颤组SIRT1表达与MDA、SOD、MT呈负相关,相关系数分别为-0.447、-0.521、-0.394(P<0.05)。结论:房颤患者右心耳组织SIRT1表达增加,与氧化应激指标呈负相关,提示SIRT1可能通过抑制氧化应激在房颤中起作用。

AIM：To observe the expression of mammalian sirtuin 1（SIRT1） in right auricle tissues in patients presenting with atrial fibrillation（AF） and make clear the relationship between SIRT1 expression and oxidative stress.METHODS： A total of 38 patients with rheumatic heart disease were divided into 2 groups： AF group（AF lasted more than 6 months,n=25） and SR（sinus rhythm） group（n=13）.The expression of SIRT1 in right auricle tissues harvested during heart operations was detected by immunohistochemistry.Oxidative stress was estimated by the amounts of malondialdehyde（MDA） and metallothionein（MT） and the activity of superoxide dismutase（SOD） which were detected using thiobarbituric acid reaction（TBA）,enzyme-linked immunosorbent assay（ELISA） and xanthine oxidase assay,respectively.RESULTS： Compared with the SR group,the expression of SIRT1 protein in the atrium significantly increased in AF group [P〈0.05,（45.8±4.03）% vs（19.7±2.54）%].In AF group,MDA was（7.24±1.05） nmol/mg,SOD（1034.25±84.32） U/mg and MT（7.21±1.46） μg/g,all being significantly higher than those in SR group[P〈0.05,MDA：（3.01±0.47） nmol/mg;SOD：（723.63±65.23） U/mg;MT：（4.31±1.23） μg/g].Spearman correlation indicated that the expression of SIRT1 had a significantly negative correlation with the amounts of MDA and MT and the activity of SOD（P〈0.05,r=-0.447,-0.521,-0.394,respectively）.CONCLUSION： The expression of SIRT1 increased in patients with AF.SIRT1 maybe effects the AF by means of inhibiting the process of oxidative stress.