MCP-1在慢性乙型肝炎合并非酒精性脂肪性肝病肝组织中的表达

Expression of MCP-1 in the patients of chronic hepatitis B complicated with nonalcoholic fatty liver disease

目的:研究慢性乙型肝炎(CHB)合并非酒精性脂肪肝病(NAFLD)患者肝组织中单核细胞趋化蛋白-1(MCP-1)表达水平。方法:CHB合并NAFLD患者21例作为实验组,单纯CHB患者46例作为对照组,采用实时定量RT-PCR方法检测肝组织中MCP-1 mRNA表达水平,免疫组化检测肝组织中MCP-1蛋白表达水平,非参数Mann-Whiteny检验比较CHB合并NAFLD患者和单纯CHB患者肝组织中MCP-1表达水平差异。结果:CHB合并NAFLD组肝组织中MCP-1 mRNA相对表达水平0.034(0.024～0.058)高于单纯CHB组MCP-1mRNA相对表达水平0.016(0.012～0.024),差异有统计学意义(P<0.01);CHB合并NAFLD组肝组织MCP-1免疫组化积分8.7±2.5高于单纯CHB组免疫组化积分(6.2±3.5),差异有统计学意义(P<0.01)。结论:CHB合并NAFLD患者肝组织中MCP-1表达水平高于单纯CHB患者。

AIM：To investigate the expression level of monocyte chemoattractant protein-1（MCP-1） in the liver of the patients with chronic hepatitis B（CHB） complicated with non-alcoholic fatty liver diseases（NAFLD）.METHODS： The study enrolled 21 CHB with NAFLD patients and 46 CHB without NAFLD patients as the controls.Real-time quantitative reverse transcriptase-polymerase chain reaction（QRT-PCR） and immunohistochemistry（IHC） were applied to detect the expression of MCP-1 at the mRNA and protein levels in the liver tissues,respectively.Non-parametric Mann-Whitney test was used to analyze the difference between the CHB patients with and without NAFLD.RESULTS： The mRNA relative expression level of MCP-1 in CHB＋NAFLD group was 0.034（0.024-0.058）,higher than that in the control group 0.016（0.012-0.024）.The immunohistochemical score was 8.7±2.5 in CHB＋NAFLD group and 6.2±3.5 in the control group.The difference in MCP-1 expression at the both protein and mRNA levels was significant statistically between the two groups（P〈0.01）.CONCLUSION： MCP-1 expression level in the liver is higher in CHB with NAFLD patients than that in CHB without NAFLD patients.