摘要
目的探讨人骨髓间充质干细胞（MSC）中血红素加氧酶1（HO-1）的表达及HO-1对MSC凋亡的影响。方法体外培养人骨髓MSC，反转录PCR（RT-PCR）检测人骨髓MSC中HO-1m RNA的表达；MSC加入HO-1特异性抑制剂锌原卟啉Ⅸ（Znpp-Ⅸ）抑制HO-1后，采用形态学观察、流式细胞术检测MSC的凋亡变化。结果人骨髓MSC中有HO-1mRNA的表达，加入HO-1特异性抑制剂Znpp-Ⅸ 12h后，倒置显微镜下观察可见MSC细胞开始出现不规则改变且胞体缩小，胞膜皱缩；凋亡细胞（AnnexinV＋／PI-）百分数高于对照MSC（61．21％±5．28％比7．48％±0．69％，P〈0．05）；细胞周期中在G0／G1峰前出现1个明显的凋亡细胞峰，凋亡细胞数高于对照MSC（21．36％±0．49％比1．02％±0．05％，P〈0．05）。结论HO-1在人骨髓MSC中表达并发挥抗细胞凋亡的作用。
Objective To explore the role of heme oxygenase-1 （HO-1） in the apoptosis of mesenchymal stem cells （MSCs）. Methods The method of reverse transcription PCR （RT-PCR） was employed to detect the expression of HO-1 mRNA in bone marrow MSCs. Morphologic observations, flow cytometry Annexin-V and DNA analysis were used to detect apoptosis and determine the content of DNA of MSCs after a 12-hour treatment of Znpp-Ⅸ, a Ho-1 special inhibitor. Results HO-1 mRNA was expressed in normal bone marrow MSCs. After culturing Znpp-IX for 12 h, MSCs showed volume reduction and membrane shrinkage. The cells of apoptotic Annexin V ＋/PI- increased significantly （61.21% ± 5.28% vs 7.48% ± 0. 69%, P 〈 0. 05 ） . The peak of apoptosis was distinct on the distribution graph of DNA （21.36% ±0.49% vs 1.02% ±0.05% ,P〈0.05）. Conclusion HO-1 plays an anti-apoptotic role in human bone marrow mesenchymal stem cells.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2012年第32期2292-2294,共3页
National Medical Journal of China
基金
军队高新技术重大项目（2010GXJS014）
甘肃省科技重大专项（1102FKDA005）
