帕金森病患者外周血T淋巴细胞亚群检测及临床意义

Changes of T lymphocyte subgroups in peripheral blood of Parkionson's disease patients

目的通过检测PD外周血T淋巴细胞亚群变化,为细胞免疫与PD的关系提供实验依据。方法通过直接免疫荧光标记法,应用流式细胞仪检测PD外周血CD3+%、CD4+%、CD8+%及CD4+/CD8+,并与健康人对照组分析。结果①PD组外周血CD3+%、CD8+%与对照组相比升高,差异有统计学意义(P<0.05),CD4+%、CD4+/CD8+与对照组相比降低,差异有统计学意义(P<0.01)。②新发PD与服用药PD外周血CD3+%、CD4+%、CD8+%、CD4+/CD8+比较差异无统计学意义(P>0.05)。③PD组外周血T淋巴细胞亚群与年龄、病程、Webster评分、Hoehn-Yahr分级分期和UPDRS-Ⅱ、Ⅲ评分均无相关性。结论①PD存在外周血T淋巴细胞亚群失衡,发病与细胞免疫功能失调有关;②PD细胞免疫状态的改变不能归于外源性多巴的摄入,神经内分泌免疫网络紊乱参与了PD的发病过程。

Objective To provide experimental evidences partly for cell immunity and Parkinson＇s disease（PD） through T lymphocyte subgroups detected in peripheral blood. Methods We detected the changes of T lymphocyte subgroups by flow cy- tonletry Results The changes of T lymphocyte subgroups ： CD3 ＋ % （64.77 ± 10. 76 ） and CD8 ＋ % （28. 24 ± 9.39 ） in PD patients are higher than those in healthy controlled group with signifieiant differences, P 〈 0.05 ; CD4＋ % （ 33.55 ±6. 84 ） and CD4 ＋/CD8 ＋ （ 1.31± 0. 55 ） are lower than those in controlled group with great significant differences, P 〈 0. 01 ; There is no sig- nificant difference between drug taken and without drug taken patients in CD3＋%, CD4 ＋%, CD8 ＋%, CD4 ＋/CD8 ＋ （P 〉 0. 05 ）. There were no correlation between the results of T lymphocyte subgroups in blood and drug intaken, age, course, Webster scale, H-Y grade and UPDRS scale. Conclusion （1）PD patients suffer from imbalance between T lymphocyte subgroups in peripheral blood; The attack of PD resulted from cell immune imbalance. （2）The changes of cell immune is not resulted from dopa- mine intaken, which suggest that PD patients＇ neuroimmune net is dysfunction.