RANTES对肥大细胞Toll样受体4表达的作用

Effect of RANTES on toll-like receptor 4 expression in mast cells

目的探讨趋化因子T细胞激活分泌调节因子(RANTES)对肥大细胞Toll样受体4(TLR4)表达的调节作用。方法将小鼠肥大细胞P815分为:RANTES 0.1、1.0、10、100ng/ml处理组(分别为A1、A2、A3、A4组)、RANTES 100ng/ml+RANTES阻断抗体10、30μg/ml组(分别为B1、B2组)以及空白对照组(C组)。作用2、6、16h后,采用流式细胞术、免疫荧光、RT-PCR检测肥大细胞上TLR4的表达情况。结果与C组相比,RANTES呈剂量依赖性地上调P815细胞中TLR4mRNA和蛋白表达(P<0.05),而B1、B2组能明显抑制该上调过程(P<0.05)。结论 RANTES上调P815细胞中TLR4的表达,从而加重过敏反应。

Objective To investigate the effect of regulated on activation,nomal T cell expressed and secreted（RANTES） on toll-like receptor 4（TLR4） expression in mast cells.MethodsThe mouse P815 mast cells were divided into seven groups of A1（RANTES 0.1 ng/ml）,A2（RANTES 1.0 ng/ml）,A3（RANTES 10 ng/ml）,A4（RANTES 100 ng/ml）,B1（RANTES 100 ng/ml plus RANTES blocking antibody 10 μg/ml）,B2（RANTES 100 ng/ml plus RANTES blocking antibody 30 μg/ml） and C（blank control）.After 2,6 and 16 h,TLR4 expression in mast cells was detected by flow cytometry,immunofluorescence and RT-PCR.Results Compared with group C,RANTES up-regulated the mRNA and protein expressions of TLR4 in mast cells in a dose-dependent manner（P〈0.05）,which was significantly inhibited in groups of B1 and B2（P〈0.05）.ConclusionRANTES may aggravate allergic response via up-regulating TLR4 expression in P815 cells.