透骨消痛胶囊含药血清对软骨细胞活性的影响

Effect of Serum Containing Tougu Xiaotong Capsule on Activity of Chondrocyte

目的观察透骨消痛胶囊含药血清对软骨细胞活性的影响。方法 SD大鼠膝关节软骨建立软骨细胞体外培养体系,第3代软骨细胞同步化后进行干预,分别确定含药血清的有效干预时间及干预浓度。各组含药血清在有效浓度作用下干预软骨细胞,在有效干预时间点采用MTT法。结果干预后,软骨细胞OD值干预72 h明显高于干预24 h、48 h、96 h(P<0.05);10%含药血清浓度组明显高于5%、20%含药血清浓度组(P<0.01)。确定72 h为有效作用时间,10%为有效作用浓度。在10%含药血清浓度的作用下干预72 h,第3代软骨细胞OD值,透骨消痛胶囊实验组明显高于空白组(P<0.01),实验2组增殖效果最明显(P<0.05)。结论透骨消痛胶囊含药血清能加速软骨细胞增殖,维持软骨细胞活性,其促进软骨细胞增殖作用与药物浓度有关,以中剂量药物浓度(含透骨消痛胶囊29 mg/d)为最佳。

Objective To observe the effect of serum containing Tougu Xiaotong capsule on the activity of chondrocyte.Methods Cartilage was isolated from the knee joint of SD rats,and used to establish a cultivation system of chondrocytes in vitro.The 3rd generation of chondrocytes were intervened after synchronization to determine the effective intervention time and intervention concentration.The chondrocytes were intervened by serum containing Tougu Xiaotong capsule under effective intervention concentration,and the proliferation of chondrocytes was observed at effective time points by MTT assay.Results After intervention of 72 h,the OD value of chondrocyte was significantly higher than that after intervention of 24 h,48 h and 96 h respectively（P〈0.05）.The OD value of chondrocyte in 10% drug-containing serum group was significantly higher than that of 5%,20% respectively（P〈0.01）.The effective intervention time was 72 h,and effective intervention concentration was 10%.After intervention of 72 h by 10% drug-containing,the OD values of chondrocyte in groups of serum containing Tougu Xiaotong capsule were significantly higher than that of blank group respectively（P〈0.01）.The proliferative effect was the most obvious in experiment group 2（P〈0.05）.Conclusion Serum containing Tougu Xiaotong capsule can promote the proliferation of chondrocyte,retain the activity of chondrocyte,and the proliferative effect is concerned with the drug concentration.In middle dose group of serum containing Tougu Xiaotong capsule,the proliferative effect is the best.