淫羊藿苷减轻大鼠肾脏缺血再灌注损伤的作用及机制研究

Protective effect of icariin on renal ischemia-reperfusion injury in rats and action mechanism

目的探讨淫羊藿苷对大鼠肾脏缺血再灌注损伤的影响及其机制。方法实验分为3组。实验组采用SD大鼠制备单侧肾脏缺血再灌注损伤模型，从术前2d开始至术后12d采用淫羊藿苷灌胃，剂量为100mg·kg^（-1）·d^（-1）；对照组也采用模型大鼠，用溶剂灌胃；假手术组仅游离。肾蒂，不用任何药物。检测术后14d内各组大鼠的体重和肾功能变化。术后24h取大鼠肾组织，检测丙二醛和氧自由基代谢酶的水平。术后3d和14d行肾组织病理学检查。结果术后3、7、11和14d，实验组大鼠肌酐清除率均高于对照组（P〈0．01）。术后3d，实验组大鼠肾小管损伤Paller评分低于对照组（P〈O．01）。术后24h，与对照组相比较，实验组丙二醛含量降低（P〈0．05），谷胱甘肽还原酶水平和还原型谷胱甘肽含量升高（P〈0．05），过氧化氢酶和双氧化物歧化酶水平升高（P〈0．05）。结论淫羊藿苷可减轻大鼠‘肾脏缺血再灌注损伤，其机制可能是通过影响氧自由基代谢酶系统而减轻氧化应激损伤。

Objective To investigate the effect of icariin on renal ischemia-reperfusion injury （IRI） and the action mechanism. Methods SD rats were divided into 3 groups. The model of unilateral renal IRI was established in SD rats, and Icariin （100 mg/kg） was orally administrated by gavage daily from 2 days before operation to 12 days after operation （icariin group）. The vehicle of icariin was administrated to IRI model rats as control group, while in the sham operation group the renal pedicel was only dissociated without treatment. Body weight and kidney function were monitored within 14 days after reperfusion. The kidney was harvested at 24 h after reperfusion, and then malonaldehyde （MDA） and activity of reactive oxygen species （ROS） scavenger enzymes were examined. Histopathological changes were observed at postoperative day （POD） 3 and 14. Results At POD 3, 7, 11 and 14, the creatinine clearance rate was significantly higher in icariin group than in control group （P〈0. 01）. Icariin group had significantly lower Paller scores which indicated tubules injury than in control group at day 3 after reperfusion （P〈0. 01）. In icariin group, MDA level was obviously decreased at 24 h after operation. Compared to control group, icariin group had statistically higher activity of glutathion reductase （GR）, catalase and superoxide dismutase （SOD）, as well as higher level of reduced glutathione （GSH） （P〈0. 05）. Conclusion Icariin has protective effects on renal IRI, and can promote recovery of kidney function. Icariin can reduce oxidative stress through increasing activity of ROS scavengers.