探讨模拟冷空气降温过程对健康大鼠和高血压大鼠凝血功能的影响

Study on the effect of cold air over the coagulation function by exposing healthy rats and hypertensive rats to a simulated cold air

目的:通过模拟冷空气温度变化过程给予健康大鼠和高血压大鼠冷刺激,以此探讨冷空气过程对机体凝血功能的影响。方法:收集张掖市2011年3月一次典型冷空气过程数据,利用气象环境模拟箱模拟其温度变化过程。将24只雄性健康大鼠和24只雄性高血压大鼠分别随机分成最低温组(Tmin组)、Tmin对照组、复温组(Tr组)和Tr对照组。将Tmin组和Tr组大鼠放入气候箱中暴露冷空气温度变化过程。在Tmin和Tr时点分别停止Tmin组大鼠和Tr组大鼠冷空气暴露,并采血以测定其凝血功能指标—凝血四项。结果:与对照组相比,健康大鼠和高血压大鼠活化部分凝血酶原时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)在降温后与对照组相比没有显著差异(P>0.05),但血浆纤维蛋白原(Fbg)含量在高血压大鼠和健康大鼠Tmin组明显高于其对照组(P<0.01)。温度恢复后,其在健康组含量仍然高于其对照组(P<0.05),而在高血压大鼠中没有差异(P>0.05)。同Fbg,反应Fbg的纤维蛋白原时间(Fbg-time)在健康大鼠Tmin和Tr组中短于对照组(P<0.01,),而在高血压大鼠中仅在Tmin组短于对照组(P<0.01)。高血压大鼠血中的Fbg含量和Fbgt明显高于和短于健康大鼠(P<0.01)。结论:①冷空气降温过程能增加机体血中Fbg含量,使凝血功能增强,可能增加心血管疾病危险性;②冷空气刺激对健康大鼠凝血功能影响强于高血压大鼠。

Objective： To explore the effect of temperature dropping process in cold air on the coagulation function both in healthy and hypertensive rats.Methods： Twenty-four male healthy Wistar rats and 24 male spontaneous hypertensive rats（SHR） were randomly divided into the minimum temperature group（Tim）,Tmin control group（Tmin-c）,recovery temperature group（Tr） and Tr control group（Tr-c）,With the simulated temperature dropping process of a cold air,collected from Zhangye city in March of 2011,the groups of Tmin and Tr were exposed to this process.Both at the Tmin and Tr,blood were collected from the rats for coagulation function measurements.Results： Compared with the control group,no significant difference was found in the results of activated partial thrombin time（APTT）,pro-thrombin time（PT） and thrombin time（TT） between any groups in any strains（P〉0.05）.The fibrinogen（Fbg） and fibrinogen-time were found to be obvious higher and shorter in Tmin and Tr of healthy rats and in Tmin of hypertensive rats in contrast to the control group.Hypertensive rats had higher level of fibrinogen and shorter level of fibrinogen-time.Conclusion： The temperature dropping process induced the increase of plasma Fbg both in the healthy and hypertensive subjects,which might be the reason to explain the higher occurrence of cardiovascular diseases event especially these activated through the formation of thrombin during cold air stress.Besides,the coagulation function of healthy subjects was more likely to be affected by cold air than the hypertensive subjects.