480米氦氧饱和潜水时粘附分子及腺苷的表达变化

Changes of adhesion molecules and adenosine in divers post 480 heliox saturation diving

目的:探讨480m氦氧饱和潜水时潜水员体内粘附分子、环磷酸腺苷(cAMP)及环磷酸鸟苷(cGMP)的表达改变。方法:4名潜水员经氦氧混合气加压至饱和深度480m,饱和逗留期间巡潜到493m。高压暴露时间共约447 h,其中加压96 h,饱和逗留49 h,减压302 h。分别在进舱前及出舱后抽取静脉血,ELISA法测定血清中的细胞间黏附分子-1(ICAM-1)、E-选择素(E-selectin)、P-选择素(P-selectin)、L-选择素(L-selectin)、cAMP、cGMP的表达变化。结果:和基础值比较,480 m饱和潜水后各潜水员血清中ICAM-1、P-selectin、E-selectin、L-selectin、cGMP均无显著改变,但cAMP由进舱前(66.72±83.15)nmol/L增高为(629.91±75.01)nmol/L(P<0.05)。结论:480 m氦氧饱和潜水减压程序是安全的,未引起减压障碍的病理生理的改变,但引起机体应激反应增强。

Objective： To investigate the changes of adhesion molecules,cyclic adenosine monophosphate（cAMP） and cyclic guanosine monophosphate（cGMP） in divers post 480 heliox saturation diving.Methods： Four divers were compressed within 96 hours to depths of 480 m with heliox-oxygen and held at the designated depth for 49 hours,excursion to 493 m during their saturation stay,then decompressed within 302 hours to the surface.The blood samples were collected before compression and post decompression,the expression level of intercellular adhesion molecule-1（ICAM-1）,E-selectin,P-selectin,cAMP,cGMP were detected with ELISA analysis box.Results： Compared with the levels of CAMs before compression,the levels of ICAM-1,E-selectin,P-selectin and cGMP in the serum were changed post decompression（P〉0.05）.The levels of cAMP were significantly elevated post decompression（629.91±75.01）nmol/L vs（66.72±83.15）（P〈0.05）.Conclusion： The decompression schedule in this heliox saturation diving is safe,the decompression sickness pathology in this diving has not been induced.But the stress response of divers are enhanced by this great depth saturation diving.