水黄皮根总黄酮对大鼠乙酸型胃溃疡胃黏膜EGF及TGF-α表达的影响

Effect of Pongamia pinnata root flavonoids on the quality of ulcer healing and expression of EGF and TGF-α in the rat model of gastric ulcer induced by acetic acid

目的:观察水黄皮根总黄酮(PRF)对胃溃疡大鼠乙酸型胃溃疡的作用,探讨该药促进溃疡愈合的可能机制。方法:采用乙酸注射至大鼠浆膜下形成胃溃疡模型,观察水黄皮根总黄酮对胃溃疡大鼠乙酸型胃溃疡愈合质量的影响,用放免法检测血清表皮生长因子(EGF)含量,用免疫组化法检测胃黏膜组织EGF、转化生长因子-α(TGF-α)的表达。结果:PRF能显著降低溃疡指数(P<0.05,P<0.01),提高血清中EGF含量(P<0.05,P<0.01),增强溃疡边缘胃黏膜EGF、TGF-α的表达(P<0.05,P<0.01)。结论:PRF能提高溃疡愈合质量,增强溃疡瘢痕处EGF、TGF-α的表达可能是其作用机制之一。

Objective： To observe the effects and mechanisms of Pongamia pinnata root flavonoids（PRF） on the experimental gastric ulcer induced by acetic acid and to study the mechanism of PRF on the quality of ulcer healing.Methods： The models were established by acetic acid erosion,the quality of ulcer healing of PRF on the model of gastric ulcer were observed.The contents of epidermal growth factor（EGF） in serum were determined by radioimmunoassay.The expression of EGF and transforming growth factor-α（TGF-α） were detected by immunohistochemistry（SP）.Results： PRF significantly inhibited ulcerative formation induced by acetic acid（P〈0.05,P〈0.01）.PRF could significantly increase the EGF and TGF-α（P〈0.05,P〈0.01） expression of para-ulcer mucosa tissue and improve the EGF contents in blood serum（P〈0.05,P〈0.01）.Conclusion： PRF increases the contents of EGF in serum and the expression of EGF and TGF-α in the tissue around gastric ulcer which might be one of possible mechanisms that PRF improves quality of ulcer healing.