SCC-9舌鳞癌肿瘤干细胞的分选和鉴定

Separation and Identification of the Cancer Stem Cells of the Tongue Squamous Cell Carcinoma Line SCC-9

目的:分离SCC-9细胞中CD44+的肿瘤细胞亚群,鉴定CD44+亚群细胞具有肿瘤干细胞的特性。方法:采用CD44免疫荧光抗体标记流式细胞术分选出CD44+的细胞亚群;检测各细胞亚群的细胞周期;检测CD133、Oct-4、BIM-1在CD44+和CD44-中的表达;检测各细胞亚群克隆形成率。结果:SCC-9细胞中CD44+的细胞亚群约占30%;CD44+的肿瘤细胞亚群70%处于G0/G1期;胚胎干细胞因子CD133、Oct-4、BIM-1在CD44+和CD44-的OSCCs中表达存在显著性差异(P<0.01);CD44+细胞亚群克隆形成率明显高于CD44-的细胞亚群。结论:在SCC-9舌鳞癌细胞系中CD44+细胞亚群具有肿瘤干细胞的特性。

Objective： To characterize the biological features of CD44＋ cell populations in oral squamous cell carcinoma（OSCC）, CD44＋ and CD44 cell populations were sorted from SCC--9 line. Methods： To sort CD44＋ subpopulation group of SCC--9 by flow cytometry. To detect the cell cycle of the CD44＋ , CD44 cell populations. To detect the expression of CD133, Oct--4, BIM--1 gene and protein, compare gent and protein expression in the CD44＋ cell population, CD44 cell population and ESCs. To detect cloning efficiency of the CD44＋ cell population, CD44- cell population. Results： The results showed that a minority population of CD44＋ cancer cells, which comprise about 30% of the cells in SCC--9. 70% CD44＋cancer cells lied in G0/G1 period. CD44＋ cells differentially expressed the CD133, Oct--4, BIM--1, ABCG2 genes, at both the RNA and protein levels, and the CD44＋ cells in the tumor expressed high levels of these nuclear genes and protein（P〈0.01）. Conclusion： CD44＋ cells had the characteristics of cancer stem--like cells in lingua squamous cell carcinoma line SCC--9.