平阳霉素作用静脉畸形内皮细胞后VCAM-1、ICAM-1、ICAM-3表达

Pingyangmycin-Regulated Expression of Vascular Cell Adhesion Molecule-1(VCAM-1),Intercellular Adhesion Molecule-1(ICAM-1) and Intercellular Adhesion Molecule-3(ICAM-3) in Human Venous Malformation Endothelial Cells

目的:研究平阳霉素(PYM)作用于人静脉畸形内皮细胞(HVMECs)后Ig粘附分子(VCAM-1、ICAM-1、ICAM-3)表达。方法:体外培养HVMECs,采用细胞ELISA和RT-PCR技术检测不同浓度PYM作用人HVMECs后Ig粘附分子表达。结果:PYM作用人HVMECs后粘附分子表达具有时间浓度效应。PYM能诱导VCAM-1在人HVMECs表达,2h后明显增高,8h后达到峰值,12h后下降,48h后呈阴性表达。PYM能促进ICAM-1在人HVMECs表达,12h后显著增高,18h最高,24h逐渐下降。PYM能促进ICAM-3表达,12h后逐渐增高,24h达到峰值,72h后1CAM-3仍高表达。0.01～1mg/L PYM能诱导或促进粘附分子表达,表达水平与药物浓度成正相关,1mg/L PYM作用人HVMECs后粘附分子表达较高,10mg/L PYM作用人HVMECs后,表达下降。RT-PCR检测PYM作用人HVMECs 2、6、12h能诱导或促进VCAM-1、ICAM-1和ICAM-3mRNA表达,8、12、18h后mRNA表达最高。1mg/L PYM作用于HVMECs后,粘附分子mRNA表达最高,mR-NA表达具有时间浓度效应。结论:低浓度PYM能诱导或促进人HVMECs粘附分子表达,具有时间浓度效应。

Objective： To investigate PYM--regulated expressions of VCAM--1, ICAM--1 and ICAM--3 in pri- mary cultured HVMECs. Methods： Expressions of the adhesion molecules VCAM-- 1, ICAM-- 1 and ICAM-- 3 were studied in PYM --regulated HVMECs in vitro by means of ELISA and RT--PCR. Results： Expressions of VCAM--1 and ICAM--3 were induced, and expression of ICAM--1 was up--regulated, both in a time and concen- tration- dependent fashion after stimulation with PYM. The expression of VCAM--1 was observed at 2h and ICAM--1 at 6h and ICAM--3 at 12h. The highest expression of VCAM--1, ICAM--1 and ICAM--3 was observed at 8h, 18h and 24h. After exposed for the same time interval, expression of adhesion molecules on HVMECs ex- posed to lmg/L of PYM was higher than that exposed to other concentration of PYM. mRNA expressions of VCAM--1, ICAM--1 and ICAM--3 started at 2h, 6h and 12h respectively. Maximal synthetic activity was oh- served at 6--8h for VCAM--1, at 12--18h for ICAM--1 and at 18--24h for ICAM--3. Synthesis activity was greatly suppressed at 10mg/L or higher concentration. Conclusion： Expression of Ig--like adhesion molecules in HVMECs can be induced or up--regulated by lower concentration of PYM in a time and concentration--dependent fashion.