摘要
从潮土、水稻土、砂姜黑土、石灰土上植物根际土壤和根中分离了86株磷细菌,通过NBRIP液体摇瓶培养3 d,培养液水溶磷质量浓度为4.2~387.3 mg.L^-1,水溶磷质量浓度与培养液pH呈显著负相关(r^2=0.621 6)。筛选出3株磷细菌进行玉米盆栽试验,结果表明,1株磷细菌处理的玉米干物质量和吸磷量与对照(处理4)相比无显著差异,2株磷细菌处理的玉米干物质量和吸磷量与对照相比有明显增加,干物质量增加了19.6%~37.5%,吸磷量增加了22.7%~40.2%,其中编号为HCW115解磷菌株的效果相当于施用无机磷(P)10 mg.kg^-1处理。
Eighty-six strains of phosphate-solubilizing bacteria(PSB) were isolated from rhizosphere soil and plant root in paddy soil,meadow soil,black clay soil and limestone soil.After 3-day incubation in a liquid medium NBRIP,soluble phosphorus(P) in the cultures of strains was 4.2-387.3 mg.L^-1,and there was significantly negative correlation(r^2=0.621 6) between pH and the soluble P in the cultures of strains.The effects of three strains of PSB(HCW036 PSB,FSJ218 PSB and HCW115 PSB) were tested in a pot experiment.The results showed that the dry weight and P uptake of corn seedling in BCW036 PSB treatment was not significantly different from the control(CK).The dry weight of corn in HCW115 and FSJ218 PSB treatments were increased by 19.6% and 37.5% compared with CK;while the P uptake were 22.7%-40.2% higher than that of CK.All the differences were statistically significant(P〈0.05).The effect of HCW115 PSB treatment on corn was almost equal to that of 10 mg P kg-1treatment.
出处
《生态环境学报》
CSCD
北大核心
2012年第7期1257-1261,共5页
Ecology and Environmental Sciences
基金
科技部星火计划项目(2010GA710048)
蚌埠市科技局项目(BK201005)
安徽科技学院重点学科项目(YZD201002)
关键词
磷细菌
土壤
玉米
phosphate-solubilizing bacteria
soil
corn seedling