系统性红斑狼疮患者外周血MMP-2、MMP-3、MMP-9和TIMP-4水平的检测及其临床意义

Detection and the clinical significance of serum MMP-2、MMP-3、MMP-9 and TIMP-4 levels in patients with systemic lupus erythematosus

目的:检测系统性红斑狼疮(SLE)患者外周血基质金属蛋白酶(MMP)-2、MMP-3、MMP-9和基质金属蛋白酶组织型抑制因子(TIMP)-4的水平,并探讨其临床意义。方法:采用双抗夹心ELISA法,检测58例SLE患者及30例正常对照者的血清MMP-2、MMP-3、MMP-9和TIMP-4水平。结果:SLE患者血清MMP-3、TIMP-4水平显著高于正常对照者,但其血清MMP-2、MMP-9水平则与正常对照者间无统计学差异。SLE患者的血清MMP-3水平与MMP-2、MMP-9、TIMP-4水平均呈正相关,MMP-9水平与MMP-2水平间亦呈正相关;同时其MMP-2、MMP-3、MMP-9水平与反映肾脏、肝脏及机体免疫状态的多组指标间存在相关性,包括尿素氮、肌酐、尿酸、白蛋白、免疫球蛋白G、免疫球蛋白A、免疫球蛋白M、补体C3和补体C4等;SLE患者中,发生血小板减少者的血清MMP-2、MMP-9水平与血小板正常者相比显著降低。结论:SLE患者外周血MMP-3和TIMP-4水平显著升高。不同MMP间可相互调节,同时也受TIMP-4等TIMP的调节。MMP-2、MMP-3和MMP-9可能参与了SLE患者肾脏和肝脏的病理损害过程。

Objective To detect serum matrix metalloproteinase （MMP）-2, MMP-3, MMP-9 and tissue inhibitor of matrix metalloproteinase （TIMP）-4 levels in patients with systemic lupus erythematosus （SLE） and define its clinical significance. Methods Serum levels of MMP-2, MMP-3, MMP-9 and TIMP-4 were measured by ELISA. Results Levels of MMP-3 and TIMP-4 were significantly higher in SLE patients than those in healthy controls. Positive correlation was found between these MMPs. Serum MMP-2, MMP-3, MMP-9 levels had a correlation with other laboratory results re- flecting the condition of kidney, liver and immunity. MMP-2 and MMP-9 levels were decreased in SLE patients having a low platelet count. Conclusins Serum MMP-2 and TIMP-4 levels are significantly higher in patients with SLE. MMP-2, MMP-3 and MMP-9 were regulated by a complicated network. TIMP-4 is involved in this network. MMP-2, MMP-3 and MMP-9 participate in the pathological process of kidney and liver impairment in SLE patients.