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前列腺素E1对1-甲脲乙醇酸酐所致肾小管上皮细胞损害的保护作用 被引量:2

Protective Effect of Prostaglandin E1 Against 1-Methylhydantoi Induced HK-2 Cell Apoptosis
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摘要 【目的】研究前列腺素E。(PGE。)对l-甲脲乙醇酸酐所致肾小管上皮细胞凋亡的保护作用。【方法】人近端肾小管上皮细胞(HK2)分三组培养,A组正常对照组;B组1一甲脲乙醇酸酐组:培养基中加入0.5mmol/L1一甲脲乙醇酸酐;C组PGEl+1一甲脲乙醇酸酐组:培养基中加入0.5mmol/L1一甲脲乙醇酸酐和PGEl2t μg/L。培养48h后用MTT比色法测光密度(OD)值,并检测培养液NAG酶(N一乙酰一p氨基葡萄糖苷酶)活性;用An—nexinv_FITC/PI流式细胞仪检测胞凋亡。【结果】B组HK~2细胞0D值较A组显著下降,NAG酶活性上升;C组较B组0D值显著上升而NAG酶活性下降;B组HK一2细胞凋亡率显著高于对照组,C组的凋亡率较B组显著下降,其差异均有统计学意义(P〈O.01)。【结论】1一甲脲乙醇酸酐有促进肾小管上皮细胞凋亡的作用。PGE。对1一甲脲乙醇酸酐所致肾小管上皮细胞凋亡有一定的保护作用。 [Objective] To investigate the protective effect of prostaglandin E1 (PGE1) against 1-mthylhydantoi induced renal tubular epithelial cell apoptosis. [Methods]Human distal renal tubular epithelial cells(HK-2) were divided into 3 groups to be cultured. Group A was normal control group. Group ]3(1-methylhydantoi group) was 0.5mmol /L 1-methylhydantoi in the medium. Group C(PGE~ + 1-methylhydantoi group) was 0.5 mmol /L 1- mthylhydantoine and 2l^g/L PGE1 in the medium. MTT method was used to detect optical density(OD) value and the activity of NAG enzyme in culture 48h after culture. Annexin V-FITC/PI flow cytometry was used to detect cell apoptosis. [Results] Compared with group A, OD value of HK-2 cells in group B significantly decreased, while NAG increased. Compare with group B, OD value in group C markedly increased, while NAG decreased. HK-2 cell apoptosis in group B was markedly higher than that in control group. Compared with group B, HK-2 cell apoptosis in group C markedly decreased. There were significant differences( P d0. 01). [Conclusion]l- Methylhydantoi can promote renal tubular epithelial cell apoptosis. PGE1 can protect renal tubular epithelial cell apoptosis induced by 1-methylhydantoi.
出处 《医学临床研究》 CAS 2012年第8期1491-1493,共3页 Journal of Clinical Research
关键词 上皮细胞/病理学 肾小管/病理学 前列地尔/药理学 Epithelial cells/PA~ kidney tubules/PA alprostadil/PD
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