酵母样真菌的浓度对UF-1000i尿沉渣分析仪检测结果的影响

The effect of yeast-like fungus diversity on the results by UF-1000i urine cell analyzer

目的探讨不同浓度的酵母样真菌对UF-1000i尿沉渣分析仪检测结果的影响。方法将白色念珠菌用无菌生理盐水制成103、104、105、106、107/mL浓度的菌悬液,UF-1000i尿沉渣分析仪分别检测菌悬液以及生理盐水对照,分析红细胞、白细胞及酵母菌结果。再取各浓度菌液,加入定量的血细胞,用同样步骤进一步验证。结果白色念珠菌的浓度为103/mL和(或)104/mL时,检测指标与对照组相比差异无统计学意义(P>0.05)。浓度为105/mL～107/mL时,酵母菌和红细胞的计数结果明显高于对照组(P<0.05),而且红细胞计数随浓度增加而递增。浓度达到107/mL时还引起白细胞计数的增高(P<0.05)。结论酵母样真菌在UF-1000i尿沉渣分析仪中的检出数量与产生干扰的数量相同,均为105/mL;大于或等于此数量可引起仪器红细胞计数假性增高;其浓度达到107/mL时,也干扰仪器白细胞计数结果。

Objective To evaluate the effect of yeast-like fungus diversity on the results by UF 1000i flow cytometer. Methods Five levels of albicans monilia suspension（range from 103/mL tol07/mL） and the normal saline control were analysed,the quantity of yeast, red blood cell and white blood cell were used to evaluated. To confirm these, the five levels of suspension were added into some quantitative blood cells, and they were analysed by same pro- cedure. Results Three indices had no difference from the control when the dose of albicans monilia was 10a/mL or 104/mL. But the quantities of yeast and RBC were significantly increased comparing the control as the doses reached to 105/mL--lOT/mL. Moreover, the amounts of RBC were increased progressively accompanied with the increasing dose. Meanwhile, as the dose reached upon 107/mL, the quantity of WBC was also significantly increased. Conclusion The detectable diversity is the same as interference-induced diversity of yeast-like fungus is 10s/mL by UF-1000i u- rine cell analyzer,the quantity of RBC is false increased in or upon this concentrition. When the density upon 107/ mL,the WBC quantity is also false increased.