3135例泌尿生殖道感染支原体的体外培养及药敏试验结果分析

Culture and analysis of drug resistance of 3 135 strains of Mycoplasma isolated in reproductive tract

目的探讨厦门市2011年1月至2012年1月支原体感染及药敏情况,为临床治疗提供依据,为流行病学调查提供资料。方法使用广东省珠海市丽珠医药公司生产的解脲脲原体和人型支原体分离鉴定、计数、药敏试剂盒对3 135例疑似支原体感染者的宫颈分泌物及男性生殖道分泌物体外培养及药敏分析。结果 3 135例疑似支原体感染者培养阳性725例(23.13%),其中解脲脲原体(Uu)阳性432例(13.78%),人型支原体(Mh)阳性162例(5.17%),Uu和Mh混合阳性131例(4.18%)。药敏分析显示,不同型的支原体对药物的敏感性不同,对美满霉素、交沙霉素、强力霉素敏感性均较高;但对克拉霉素、罗红霉素、阿奇霉素、氧氟沙星等敏感性同感染支原体类型有相关性。结论泌尿生殖道支原体感染者体外培养及药敏分析对指导临床合理选用敏感药物,减少耐药菌株产生具有重要意义。

Objective To discuss situation of mycoplasma infection and drug sensitivity in Xiamen City from August 2008 to April 2009, and to provide the aid for clinical treatment and information for epidemiological investiga- tion. Methods 3 135 isolations of Ureaplasma urealyticum and Mycoplasma hominis from suspected infections of cer- vical secretion and male reproductive tract were cultured and detected for sensitivity analysis by identification, count- ing, sensitivity kit produced by Livzon Pharmaceutical company in Zhuhai City, Guangdong Province. Results 725 （23. 13~） Mycoplasma infection strains were isolated for 3 135 specimens, among which there were are 432 （13.78%） strains infected by Ureaplasma urealyticum（Uu） and 162（5.17%） strains infected by Mycoplasma homi- nis（Mh） ,the others（131/4. 18%） were UU and MH mixed positive. The susceptive analysis was showed that the drug sensitivities of different biological types of Mycoplasma were different, the highest susceptive antibiotics were Doxycycline（DOX）, Minocycline（MIN） and Josamycin（JOS）. However, other drugs such as Clarithromycin （CLA）, Roxithromycin（ROX）, Azithromycin（AZI）,Ofloxacin（OFL）, etc. had the relevance with the biological types of My- coplasma. The highest resistant drug was Levofloxacin（LEV）, then were Ofloxacin（OFL） and Sparfloxacin（SPA）. Conclusion Culture of Mycoplasma in vitro and analysis of drug resistance have the great significances for clinical appliance in drugs, and reducing drug-resistant strains.