反相高效液相色谱法测定清肝助眠汤中芍药苷的含量研究

Determination of Paeoniflorin in Qingganzhumian Decoction by RP-HPLC

目的建立以反相高效液相色谱(RP-HPLC)法测定清肝助眠汤中芍药苷含量的方法。方法色谱柱Agilent Technologies C18(250mm×4.6mm,5μm),流动相为甲醇-1%磷酸(30∶70),流速为1.0ml/min,柱温为25℃,检测波长为230nm。结果芍药苷检测量在0.34～0.17μg范围内与峰面积有着线性关系(r=0.9999);平均加样回收率为100.04%,RSD=0.78%(n=9)。结论该方法方便易行,结果准确,重复性好。

[ Abstract] Objective To establish a reversed phase high -performance liquid chromatography （RP -HPLC） method for the determination of paeoniflorin concentration in Qingganzhumian Decoction. Methods The Agilent technologies C - 18 （250mm ×4. 6mm, 5μm） column was used with the mobile phase consisted of methanol - 1% phosphoricaeid （30： 70） at the flow rate of 1.0ml/min. The temperature of column was 25℃, and detection wavelength was set at 230nm. Results It showed a good linear relation between Paeoniflorin content and peak area at a range of 0. 34 - 0. 17 μg （r = 0. 9999 ）, and the average re- covery rate was 100. 04% （ RSD = 0. 78%, n = 9）. Conclusion The RP - HPLC method for the determination of paeoniflorin concentration is simple, accurate and highly reproducible.