气相色谱指纹图谱法快速鉴别红葡萄酒真假

Research on Rapid Identification of True or False Red Grape Wine Based on GC Fingerprints

通过采集10个常见葡萄酒产地中85份真红葡萄酒样品的气相色谱指纹图谱,利用中药色谱图分析和数据管理系统软件进行识别分析,建立了红葡萄酒的气相色谱指纹图谱鉴别葡萄酒真假方法。结果表明,该方法过程仪器的精密度和方法的重现性很好,真红葡萄酒标准指纹图谱包括12个特征色谱峰,其气相色谱指纹图谱峰面积相关系数法、峰面积夹角余弦法、全图谱相关系数法和全图谱夹角余弦法的相似度阈值为≥0.9700。3种方式勾兑的假葡萄酒的试验表明,掺水稀释葡萄酒峰面积的相关系数法和夹角余弦法相似度值都在0.1700以下;葡萄汁勾兑葡萄酒峰面积相关系数法和夹角余弦法相似度值在0.6128以下;人工色素勾兑假葡萄酒峰面积相关系数法和夹角余弦法的相似度值在0.1001以下。通过市售2种可疑葡萄酒的验证试验表明,该2种可疑葡萄酒的峰面积相关系数法和夹角余弦法的相似度值在0.1500以下,低于真红葡萄酒的相似度阈值;且缺少红葡萄酒具有的特征色谱峰,可确定为假葡萄酒。本方法操作简便、快速、客观、准确,是一种值得推广应用的葡萄酒真假的鉴别方法。

A method had been developed for rapid identification of true or false red grape wine based on GC fingerprint technology. 85 GC finger-prints of red grape wine from ten countries were selected and analyzed, and all data were calculated by using chromatographic analysis and data management system of Chinese traditional medicine. In the whole process, the precision of instruments and the repeatability of the method were good, the standard fingerprints of true red grape wine owned 12 characteristic GC peaks, and the similarity threshold of all 85 samples was no less than 0.9700 through analyzing peak area correlation coefficient （r）, peak area cosine ratio （Cos a ）, total chromatography correlation coefficient （t r）, and total chromatography cosine ratio （t Cos a ） respectively. The similarities of adulterated red grape wine （including water mixing, grape juice mixing and pigment-added red wine） were as low as 0.1700,0.6128 and 0.1001 by use of analyzing peak area correlation coefficient （r） and peak area cosine ratio （Cos a ）. Two kinds of red grape wine bought from supermarket and analyzed by using such method, the analytical results demonstrated that the similarity was 0.1500, lower than the threshold of true red wine, and their fingerprints absent characteristic peaks of true red grape wine. Thus, they were identified as false red grape wine. Such method was simple, rapid, objective and accurate, and worthy of popularized application.