糖尿病血管内皮细胞损伤与β淀粉样肽的相关性研究

Relationship of amyloid-β with vascular endothelial cell damage induced by diabetic serum

目的探讨糖尿病（DM）血管内皮细胞损伤与β淀粉样肽（Aβ）的相关性。方法选取2型糖尿病患者（DM组）和健康对照（HC组）各10例，利用其血清对人脐静脉内皮细胞株（HUVEC）进行干预，同时胎牛血清作为对照组（FBS组），检测干预72h时内皮细胞生存率，干预0．5、3、72h时上清液中超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）、AB40以及A[342的含量。结果DM患者A1340较健康对照者明显升高（P〈0．01）；上清液中A1340[0．5、3、72h时，DM组分别为（163±64）、（207±69）、（286±75）ng／L，HC组分别为（146±76）、（154±75）、（161±72）ng／L]以及A阴2的水平[0．5、3、72h时，DM组分别为（48±46）、（54±43）、（79±44）ng／L，HC组分别为（41±12）、（44±16）、（49±12）ng／I。]，组别与干预时间均有交互作用（P〈0．05）。HC与FBS组随着干预时间的延长，DM组SOD和NO水平，呈下降趋势，72h时最低，而HC和FBS组呈上升趋势，72h时最高；Pearson相关分析显示，在干预72h时SOD与A1340和AB42呈负相关（r=-0．482、-0．422，P〈0．05）；MDA与AIM0和A1342呈正相关（r=0．418、0．833，P〈0．01）。结论DM血管内皮细胞氧化应激损伤与A1340、A1342有关。

Objective To explore the relationship of the impairment of human umbilical vein endothelial cell （HUVEC） with amyloid-β, Methods HUVECs were cultured in the serum of patients with type 2 diabetes mellitus （DM） or serum of healthy control （HC）, while fetal bovine serum （FBS） was used as a negative control. The proliferative activity of HUVEC were assessed by thiazoyl blue tetrazolium bromide （MTT） after 72 h. The supernatant concentrations of superoxide dismutase （SOD） , maleic dialdehyde （MDA）, nitric oxide （NO）, amyloid-1340 （Aβ40） and Aβ42 were measured after 0. 5, 3 and 72 h respectively. Results Glycosylated hemoglobin values, fasting plasma glucose and fasting plasma Aβ40 concentrations of diatbetie patients were higher than those of healthy counterparts （ P 〈 0. 01 ）. Proliferative activity of HUVECs in group DM were significantly lower than that of group HC. Both group and the time of intervention had crossover effects on the levels of MDA, SOD, NO and A1340 （ （163±64）, （207±69）, （286±75） ng/L in group DM; （146±76）, （154±75）, （161±72） ng/L in group HC after 0.5, 3 and 72 h, P〈0.05） andAβ42 （（48±46）, （54±43）, （79±44） ng/LingroupDM; （41±12）, （44±16）, （48±12） ng/L in group HC after 0.5, 3 and 72 h, P 〈0.05）. With the elongating time of intervention, the levels of SOD and NO decreased significantly in group DM and reached the lowest after 72 h while increased significantly in groups HC and FBS and peaked after 72 h. The concentrations of MDA, Aβ40 and Aβ42 increased significantly in all three groups while the fastest and marked increments were found in group DM （P 〈0. 01 ）. Pearson correlation analysis showed that SOD was negatively correlated with Aβ40 （ r = - 0. 482, P = 0. 02 ） and AI342 （ r = - 0. 422, P = 0. 02） while MDA positively with Aβ40 （ r = 0.418, P〈0.05） and AI342 （r=0. 833, P〈0.05） after 72 h. Condusion Oxidative stress of vascular endothelial cells may be correlated with Aβ40 and Aβ42 in diabetes.