氨基寡糖素发酵生产的研究

Study on Processing of Amino Oligomers

利用假单胞菌进行了发酵生产氨基寡糖素的研究，结果表明发酵生产的最佳条件是：含0．25％壳聚糖的基础发酵培养基中添加0．05％的吐温20，培养基装量125mL（250mL三角瓶），28℃120r／min振荡培养发酵84h。重复验证试验证明产量稳定在4．5μmol／mL左右，产率达31％左右。

Amino oligomers were produced by fermentation of Pseudomonas sp. VIIIT39. The optimal composition(g/L) of the medium at a flask scale was: chitosan 2.5, MgSO4.7H2O 0.5,KH2PO4 0.5, KCl 0.5, FeSO4.7H2O 0.01and Tween20 0.5mL.Optimal temperature and initial pH for production were 28℃ and 6.5 respectively. When Pseudomonas sp. VIIIT39 was cultivated on arotary shaker(120r/min) at 28 ℃ for 84hrs. in a 250mL flask containing 125mL of medium,the results showed that 4.5 μ mol/mL of amino oligomers were accumulated(31% yield from chitosan).