稻谷中苯并(a)芘的测定

Determining the BaP Concentration Paddy

将粉碎后的稻谷样品置于脂肪提取器中，采用石油醚回流提取8h。提取液用中性氧化铝净化，KD浓缩器浓缩。采用液相色谱对稻谷中苯并（a）芘的含量进行测定，分离柱ODS-C18；流动相为85％，甲醇和15％水，流速为1ml／min；荧光检测器检测。

This experment was conducted by following the procedure of a method which could determine the BaP concertration in paddy by liquid chromatograph. First, broke the paddy sample into piece,then put these pieces into a fatextractor. And secondly.recirculated petroleum ether extraction for 8 hours.Neufnal aluminas,were used fou the cleaning of extracted solutions with KD concentration for enrichment.At last,Liquid Chromatograph was used to determine the BaP concentration. The working conditions. of LC were as follows: separate coloum was ODS-C18. mobile phase was a solution of 85%CH3OH and 15%H2O with flow rate as 1 ml/min and monitored by fluorescent light.