奶牛不同发育时期乳腺细胞凋亡规律的研究

Study on apoptosis of bovine mammary gland in different development stages

为系统地研究奶牛乳腺不同发育时期细胞凋亡的规律,应用TUNEL法对奶牛不同发育时期正常乳腺组织(冰冻切片)的乳腺细胞凋亡进行了系统检测。结果表明,青春期乳腺发育缓慢,结构变化较小,乳腺细胞凋亡量相对较少;妊娠期乳腺导管持续发育,凋亡量上升,其中妊娠初期2月,乳腺腺泡大量出现,脂肪细胞凋亡随之增加,出现了一个高峰;泌乳期乳腺的结构和功能最为完善,乳腺结构变化很小,细胞凋亡量维持在很低水平;退化期腺泡瓦解,大量细胞发生凋亡,其中退化初期乳腺细胞凋亡持续增加,退化3 d达到最大值,之后凋亡量逐渐降低,退化30 d后乳腺已经基本恢复到青春期状态,凋亡细胞量也随之减少。

The purpose is to explore the regulation of apoptosis at different developmental stages in dairy cow mammary gland. Normal cow mammary gland tissues of different developmental stages were sliced in frozen. TUNEL was used to detect the apoptosis of mammary cell. The results showed that the virgin mammary gland developed slowly, little changes in mammary gland, and at this time, no apoptosis was detected. In pregnancy, mammary gland ductal continued to grow, and apoptosis increased. In initial pregnancy 2m, there were a lot of gland alveolus and simultaneously adipocyte apoptosis began to increase, and appearing a secondary apoptosis peak. In lactation, the structure and functibn of mammary gland were most complete, no changes in mammary gland structure and apoptosis level was low. In involution, gland alveolus co/- lapsed and a lot of cells were apoptosis. In involution 3d, the apoptosis was highest and then decreased. In involution 30d, the mammary gland was recovered and apoptosis decreased.