川楝子乙醇部位HPLC指纹图谱研究

HPLC Fingerprints of Ethanolic Part of Toosendan Fructus

目的建立川楝子乙醇提取部位的HPLC指纹图谱。方法色谱柱Agilent zorbax SB-C18柱(4.6 mm×150 mm,5μm);流动相甲醇/乙腈(1∶10)(A)-0.4%磷酸溶液(B),梯度洗脱;检测波长360 nm;柱温25℃;流速1.0 mL.min 1。结果建立了川楝子药材乙醇提取部位的HPLC色谱指纹图谱。在指纹图谱中共有峰20个,并指认了3个色谱峰。结论该方法准确可靠,重复性好,可为川楝子药材及其饮片的质量控制提供参考依据。

OBJECTIVE To establish HPLC fingerprints of ethanolic extraction part of Toosendan Fructus.METHODS Agilent zorbax SB-C18 column（4.6 mm×150 mm,5 μm） was adopted.A gradient elution was applied with mobile phase A（methanol and acetonitrile 1∶10） and mobile phase B（0.4% phosphoric acid solution）.The flow rate was 1.0 mL.min 1,the detective wavelength was 360 nm,and the column temperature was 25 ℃.RESULTS HPLC fingerprints of ethanolic extraction part of Toosendan Fructus was established.Twenty peaks were observed on the HPLC fingerprints of Toosendan Fructus and 3 of them were identified.CONCLUSION The method developed in this study is accurate with high repeatability,and available for the quality control of Toosendan Fructus and its decoction pieces.