二烯丙基一硫化物对小鼠急性酒精性肝损伤的保护作用

Protective effects of diallyl sulfide against acute ethanol-induced liver injury

目的研究二烯丙基一硫化物(DAS)对急性酒精性肝损伤的保护作用。方法将40只健康SPF级雄性昆明小鼠随机分为4组,分别为对照组、模型组、DAS干预组和DAS对照组,每组10只。DAS干预组和DAS对照组小鼠经口灌胃给予50 mg/kg的DAS溶液,每天1次,连续7 d;对照组和模型组小鼠给予等体积的玉米油。末次给药2 h后,模型组和DAS干预组小鼠经口灌胃给予5 g/kg乙醇诱导肝损伤,连续3次,每次间隔12 h;对照组和DAS对照组小鼠给予等体积等热量的麦芽糖糊精。测定血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的活力以及肝组织匀浆中谷胱甘肽(GSH)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPx)活力。结果与模型组比较,对照组、DAS干预组和DAS对照组小鼠血清ALT、AST活力和肝组织匀浆中MDA含量均较低,肝组织匀浆中GSH含量和SOD、GPx、GR活力均较高,差异有统计学意义(P<0.05或P<0.01)。结论 DAS对小鼠急性酒精性肝损伤有保护作用,机制可能与其具有的抗氧化活性有关。

Objective To study the protective effects of diallyl sulfide（DAS） against acute ethanol-induced liver injury in mice. Methods A total of 40 SPF male Kunming mice were randomly divided into four groups, control group, ethanol group, DAS plus ethanol group, and DAS group （n=10）. The mice in DAS plus ethanol group and DAS group were pretreated with DAS （50 mg/kg body weight, i.g., 10 ml/kg） for seven consecutive days, while the mice in the other groups were treated with the stone volume of corn oil. Two hours after the last treatment, the mice in ethanol group and DAS plus ethanol group were treated with 5 g/kg body weight ethanol （20 ml/kg bodyweight,,i.g.） for total of three doses with an interval of 12 h,while the other mice were treated with isocaloric and isovolumetric maltose-dextrin. The serum aminotransferase （ALT, AST） activities and the hepatic malondialdehyde （MDA）, glutathione （GSH）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, and glutathione reductase （GR） activities were determined. Results Compared with the model group, the serum ALT and AST activities and hepatic MDA content in mice of ethanol group were significantly increased,while the hepatic GSH content and the activities of SOD, GPx, and GR, were significantly decreased （P〈0.05, P〈0.01 ）. Conclusion DAS may protect mice against the acute ethanol-induced liver damage,which may be associated with its antioxidant capacity.