高效液相色谱-荧光法测定水果蔬菜中的乙萘酚

Determination of 2-naphthol in fruits and vegetables by high performance liquid chromatography with fluorescence detection

建立了柑橘、苹果、菠菜、西红柿等水果和蔬菜中乙萘酚的高效液相色谱-荧光快速检测方法。样品用乙腈提取,浓缩,乙腈-甲苯(3:1,V/V)溶解后经LC-NH2固相萃取柱净化;色谱柱:Pickering C18(250 mm×4.6 mm,5μm);流动相:乙腈-水(50:50,V/V);检测波长:λex=250 nm,λem=410 nm。乙萘酚在0.20～50.00 mg/L范围内线性关系良好(r=0.9999);在0.05,0.10,0.50 mg/kg 3个添加水平下平均回收率范围在83.3%～101.2%之间,相对标准偏差为4.3%～9.3%。

An analytical method for the determination of 2-Naphthol in fruits and vegetables by high performance liquid chromatography with a fluorescence detector was developed. The samples were extracted with acetonitrile, and the extract was concentrated and redissolved with acetonitrile-toluene （3：1, V/V）, then cleaned up with LC-NH2 cam4dge, and separated with a Picketing Clscolmnn （250 mm ×4. 6 mm, 5 μm）, acetonitrile-water （50：50,V/V） as mobile phase with isocratie elution, detected with a fluorescence detector and the excitation and emission wavelengths were 250 nm and 410 nm. 2-Naphthol had good linear relationship in the range of O. 50 -50. 00 mg/L, the correlation coefficient was higher than 0. 9999; Average recoveries of 2-Naphthol at the concentrations of 0. 05, 0. 10, 0. 50 mg/kg were 83. 3% - 101.2% and relative standard deviations were 4. 3 % -9. 3 % .