miR-21在宫颈鳞癌淋巴结转移患者血清中的表达及其临床意义

Expression of miR-21 in squamous cell carcinoma of cervix with lymph node metastasis and its clinical significance

目的:探讨miR-21在宫颈鳞癌患者血清中的表达及其与淋巴结转移的相关性。方法:选择2011年1-8月在广西医科大学附属肿瘤医院妇瘤科住院接受治疗的74例患者为研究对象,其中,宫颈鳞癌伴淋巴结转移者23例,宫颈鳞癌不伴淋巴结转移者39例,正常对照组12例,应用茎环实时荧光逆转录聚合酶链反应(real-time RT-PCR)检测血清标本中miR-21表达情况。采用多个独立样本的非参数检验分析,对其中任意两组的比较采用秩和检验分析。结果:3组miR-21表达水平差异有显著性(P<0.05),对3组进行两两比较,宫颈鳞癌伴淋巴结转移组的血清miR-21表达水平较不伴淋巴结转移组及正常对照组升高(P<0.05),差异有统计学意义(P>0.05),宫颈鳞癌不伴淋巴结转移组血清中miR-21表达水平同正常对照组比较,差异无统计学意义(P>0.05)。结论:血清miR-21在宫颈鳞癌淋巴结转移预测方面有一定的临床价值。

Objective To explore the expression of miR-21 in squamous cell carcinoma of cervix and the correlation with lymph node metastasis （LNM）. Methods The patients of 74 women were obtained from January 2011 to August 2011 in Guangxi medical university cancer hospital, applied hairpin of real-time RT-PCR to detect the expression level of serum miR-21.23 cases were squamous cell carcinoma of cervix with LNM, 39 cases were squamous cell carcinoma ~f cervix without LNM, 12 cases were normal control. Applied non-parametric test for multiple independent samples, each two group with rank sum test. Results There were statistically significant correlations between the three groups （P 〈 0.05）. The serum miR-21 level of cervical cancer cases with LNM was higher than the other two groups. There was no statistical significance between cervical cancer without LNM and normal control group. Conclusion Serum miR-21 may have its clinical value in predicting LNM in cervical cancer.