摘要
以热带水果菠萝皮为原料,采用漂烫灭酶、酸法提取和醇沉工艺提取果胶,对提取果胶的分离纯化和组成进行研究。结果表明:菠萝皮果胶经离子交换层析后分离纯化出1个中性组分,命名为PRP-N,2个酸性组分,命名为PRP-1、PRP-2。比对PRP-1、PRP-2的组成和结构发现,PRP-1的半乳糖醛酸含量较低,中性糖含量较高,单糖成分主要为鼠李糖、半乳糖和阿拉伯糖,以鼠李糖半乳糖醛酸Ⅰ型(RG-Ⅰ)结构为主,侧链主要成分为阿拉伯糖和半乳糖,通过4-O-Rha连接到主链上;PRP-2的半乳糖醛酸含量高,中性糖含量低,以半乳糖醛酸型(HG)和鼠李糖半乳糖醛酸Ⅱ型(RG-Ⅱ)结构为主,含有少量RG-I型果胶分子结构。PRP-1比PRP-2的流体力学体积小,且聚合度分布更集中。
A pectin was prepared by blanching, acid extraction, and alcohol precipitation from tropical pineapple rind. Its purification and composition were also analysed. The results showed this pectin could be completely fractionated into one neutral fraction （PRP-N） and two acidic fractions （PRP-1 and PRP-2） by ion-exchange chromatography. PRP-1 was mainly rhamnogalacturonan type I, which contained more neutral sugars than galacturonic acids. The neutrual sugars were comprised of rhamnose, galactose and arabinose, where galactose and arabinose connected to rhamnose through 4-O-Rha. For PRP-2, it contained more galacturonic acids than neutral sugars. It＇s structure contained homogalacturonan as well as rhamnogalacturonan type II with a very low amounts of rhamnogalacturonan type I. In addition, PRP-1 showed a smaller hydrodynamic volume and narrower distribution than PRP-2.
出处
《热带作物学报》
CSCD
北大核心
2012年第8期1476-1481,共6页
Chinese Journal of Tropical Crops
基金
国家自然科学基金(No.31260386)
国家重点实验室目标导向项目(No.SKLF-MB-201004)
关键词
菠萝皮
果胶
纯化
组分分析
Pineapple find
Pectin
Purification
Monosaccharide
