非小细胞肺癌LUNX mRNA的表达及临床意义

Expression and clinical significance of LUNX mRNA in non-small cell lung carcinoma

目的探讨非小细胞肺癌(NSCLC)患者外周血中肺特异性X蛋白(LUNX)mRNA的表达及其临床意义。方法采用逆转录-聚合酶链反应(RT-PCR)方法检测56例NSCLC患者(实验组)及34例肺良性病变患者(对照组)外周血中LUNXmRNA的表达情况,分析其与临床病理组织学特征的关系。结果①实验组NSCLC患者外周血中LUNXmRNA阳性表达率为58.93%,而对照组无表达(P<0.05)。②NSCLC患者外周血LUNXmRNA阳性表达率与肿瘤淋巴结转移、分化程度和临床分期有关(P<0.05),而与患者年龄、性别、吸烟以及肿瘤部位、病理类型等均无关(P>0.05)。结论外周血LUNXmRNA的检测可作为评价NSCLC发生、发展潜在指标,对判断病程和指导治疗具有重要的临床意义。

Objective To explore the expression and clinical significance of lung specificity protein X （LUNX）mRNA in peripheral blood of patients with human non-small cell lung carcinoma（NSCLC ）. Methods Reverse transcription-polymerase chain reaction（RT- PCR） was used to measure the expression of LUNX mRNA in peripheral blood of 56 NSCLC patients and 34 patients with benign lung lesions.The relationship between LUNX mRNA expression levels and clinicopathological features was analyzed. Results （~LUNX mRNA was expressed in 58.93% of NSCLC patients,while no expression was detected in patients with benign lung lesions（P〈0.05 ）. （2）LUNX mRNA expression correlated closely with lymph node metastasis and with the degree of differentiation and clinical stage of NSCLC（P〈0.05）.However,no correlation was observed between LUNX mRNA expression and other clinicopathological features such as age,sex,smoking history,tumor location or pathology type（P〉0.05）. Conclusions LUNX mRNA may serve as an important molecular indicator for evaluating the occurrence and development of NSCLC patients, and it may play an important role in clinical diagnosis and therapy.