利用SSR分析山西省玉米地方品种的遗传多样性

Analysis of Genetic Diversity of Maize Landraces in Shanxi by SSR Markers

采用混合取样方法和SSR分子标记技术,利用48对引物对山西省38个玉米地方品种的遗传多样性进行了分析。共检测出368个等位基因,每个SSR位点的等位基因数为2～14个,平均为7.48个;多态性信息量(PIC)变化范围在0.24～0.89之间,平均为0.66。总共检测出185个稀有等位基因,21个特有等位基因。SSR标记聚类分析把38个品种大体分成了4个群。研究表明,山西地方品种遗传多样性非常丰富,很多品种具有频率很高的独特基因,它们可能具有一定的特异性。因而,山西玉米地方品种对于拓宽玉米种质的遗传基础可能会起很大的作用。

The genetic diversity of maize landraces in Shanxi was studied based on SSR markers and bulk sampling method.Totally 368 alleles were detected in 38 maize landraces with an average of 7.48 and range of 2~14 per locus.PIC among these cultivars ranged from 0.24 to 0.89.Also,185 rare alleles and 21 unique alleles were identified.These landraces were clustered into 4 groups.The results suggested that genetic diversity of maize landraces in Shanxi are very rich and they contains many unique alleles that means they have some special characterization.The maize landraces in Shanxi may play a great role in broadening the genetic bases of germplasm in maize.