甘蔗与斑茅割手密复合体杂交后代的分子标记鉴定

Identification of Progeny from Crosses between Sugarcane(Saccharum spp.)and Intergeneric Hybrid Complex(Erianthus arundinaceus×Saccharum spontaneum)with Molecular Markers

为有效利用甘蔗野生种质拓宽甘蔗遗传基础,本研究利用甘蔗与斑茅割手密复合体进行杂交,同时应用序列相关扩增多态性(SRAP)和微卫星(SSR)分子标记技术鉴定其后代。两种分子标记鉴定结果相互印证表明:3个杂交组合的后代中,经表型鉴定含斑茅血缘的34个后代为真杂种。该真杂种后代为进一步综合利用斑茅、割手密的优异基因改良甘蔗品种提供了优良的创新种质。

To get potential gene resources from wild germplasm for broadening sugarcane genetic base,crosses between sugarcane（Saccharum spp.）and intergeneric hybrid complex（Erianthus arundinacius×Saccharum spontaneum）were carried out and their progenies were then identified by microsatellites（SSR）and sequence-related amplified polymorphism（SRAP）molecular markers in this study.34 true hybrids were identified and the results confirmed that utilizing both S.spontaneum and E.arundinaceus in introgression breeding programs to improve sugarcane was viable.