二烯丙三硫对人前列腺癌PC-3细胞生物学行为的影响及与uPA系统关系的初探

Effects of Diallyl Trisulfide on the Biology of Human Prostate Cancer PC-3 Cell Line and Its Relationship with uPA System

目的:研究二烯丙三硫(DATS)对人前列腺癌PC-3细胞生物学行为的影响及对尿激酶型纤溶酶激活物(uPA)系统的调节作用。方法:细胞增殖活性(MTS)比色法测定20、40、60μmol·L-1DATS处理前列腺癌PC-3细胞24、48、72h后PC-3细胞的增殖能力;细胞体外侵袭试验检测20、40、60μmol·L-1DATS对PC-3细胞侵袭能力的作用;划痕试验观察40μmol·L-1DATS处理PC-3细胞24h后PC-3细胞迁移能力;逆转录聚合酶链式反应(RT-PCR)和蛋白印迹(Western blot)法分别检测40μmol·L-1DATS作用PC-3细胞后的uPA、uPAR和PAI-1的mRNA及其蛋白表达水平。结果:20、40、60μmol·L-1DATS对PC-3细胞的生长均有较明显的抑制作用,且呈浓度和时间依赖性;DATS呈浓度依赖抑制细胞的浸润能力,其中40μmol·L-1与体内试验更接近;40μmol·L-1DATS明显抑制细胞的迁移能力,并在mRNA和蛋白水平上,显著上调PAI-1的表达,但对uPA和uPAR的表达无显著影响。结论:DATS抑制PC-3细胞的增殖、浸润和迁移,该效应可能与DATS显著上调PAI-1的表达有关系。

OBJECTIVE： To investigate the effects of diallyl trisulfide （DATS） on the biology of human prostate cancer PC-3 cells of people and the regulation to urokinase plasminogen activator （uPA） system. METHODS： Prostate cancer PC-3 cell line was treated for 24, 48, 72 h with various concentrations of DATS （20, 40, 60 μmol.L^-1）, and then MTS assay was used to determine its cellular proliferation; matrigel invasion assay was performed to assess the effects of DATS （20, 40, 60 μmol.L^-1） on the inva- sive capacity of PC-3 cells; wound-healing assay was used to analyze the motility of PC-3 cells by 40 μmol. L^-1 DATS treatment for 24 h; RT-PCR and Western blot were adopted to detect mRNA and protein expression levels of uPA, uPAR and PAI-1 in PC-3 cells after 40 μmol. L^-1 DATS treatment. RESULTS： 20, 40, 60 μmol- L^-1 t DATS significantly inhibited the proliferation of PC-3 cells in a dose and time-dependent manner. DATS inhibited invasiveness and motility capacity of PC-3 cells in dose-dependent man- ner, PAI-1 was significantly induced by 40 μmol.L^-1 DATS at mRNA and protein expression level, whereas uPA and uPAR expres- sion levels were not. CONCLUSION： DATS significantly inhibit proliferation, invasiveness and motility of PC-3 cell line. The ef- fect is possibly associated with up-regulation of PAI-1 by DATS treatment.