尼莫地平对弥散性轴索损伤大鼠血脑屏障通透性的影响研究

Effects of Nimodipine on Blood-brain Barrier of Rats with Diffuse Axonal Injury

目的:研究尼莫地平对弥散性轴索损伤(DAI)大鼠血脑屏障(BBB)通透性的影响。方法:取大鼠随机分为DAI组和尼莫地平组,每组40只,2组大鼠建立DAI后,尼莫地平组腹腔注射给药0.5mg·kg-1·d-1,分别于建模前和建模后0.25、1、3、7d检测脑组织含水量和伊文蓝(EB)含量以考察脑水肿情况和BBB通透性变化。结果:DAI后,2组大鼠脑组织含水量和EB含量均呈先升高后降低的趋势,峰值分别出现在建模后1d和3d;与DAI组比较,尼莫地平组脑组织含水量和EB含量的峰值均明显降低(P<0.05)。结论:尼莫地平可以有效减轻DAI后脑水肿情况,降低BBB的通透性。

OBJECTIVE： To study the effects of nimodipine on blood-brain barrier （BBB） of rats with diffuse axonal injury （DAI）. METHODS： Rats were randomly divided into DAI group and nimodipine group with 40 rats in each group. After model- ing, nimodipine group was intraperitoneally injected nimodipine 0.5 mg. kg-1. d-1. The brain water content and the EB content were determined before and 0.25, 1, 3, 7 d after modeling to investigate the changes of cerebral edema and permeability of BBB. RE- SULTS： After modeling, brain water content and EB content of 2 groups increased first and then decreased; the peak value of them appeared at 1 d and 3 d after modeling. Compared with DAI group, peak value of brain water content and the EB content were significantly lowered（P〈0.05）. CONCLUSION： Nimodipine decreases the permeability of BBB and has definitive effect on brain edema after DAI.