20E对急性肝损伤模型小鼠抗氧化作用研究

Anti-oxidative Ability Study of 20-hydroxyecdysone on Acute Liver Injury of Mice

目的:为了探讨20-羟基蜕皮甾酮(20E)对四氯化碳(CCl4)急性肝损伤的保护作用及相关机制。方法:小鼠体重(20±2)g随机分成3组,20E治疗组皮下注射2mg.kg-1的20E注射液,正常组和模型组注射等量的生理盐水。给药7天后,模型组和20E治疗组腹腔注射剂量为10mL.kg-1的0.25%CCl4大豆油溶液,正常组注射等体积大豆油,摘除眼球取血,制备血清,解剖肝脏。测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)的含量,采用real-time PCR测定过氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)和肿瘤坏死因子(TNF-α)的表达。结果:20E处理之后血清ALT、AST和ALP含量分别比模型组降低了31.3%、35.2%和50.7%(P<0.05);肝脏的组织学病变较模型组显著减轻;SOD和GSH-PxmRNA水平分别比模型组升高84.5%(P<0.01)和105.3%(P<0.01),ACT和TNF-α分别减少了53.7%(P<0.05)和71.2%(P<0.05)。结论:20E对小鼠急性肝损伤有显著的保护作用,其机制可能与增强肝组织抗氧化能力有关。

Objective ： To study the 20E protecting-mechanism in acute liver injury induced by carbon tetrachloride （ CC14 ）. Methods ： The mice, （ 20 ± 2 ） g weight, were randomly divided into three groups. The 20E treatment group was injected of 20E solution at a concentration of 2rag . kg^-1 and with similar volume saline solution in normal group and model group, respectively. On the 7th day, model group and 20E treatment group was injected 10mL . kg^-1 with a concentration of 0.25% CC14, normal group with similar volume soybean oil. The expressions of alanine aminotransferase （ ALT ）, aspartate aminotransferase （ AST ） and Alkaline phosphatase （ ALP ） were analyzed by automatic biochemical analyzer in serum. The real-time PCR was used to measure the expressions of superoxide dismutase （ SOD ）, catalase （ CAT ）, glutathione peroxidase （ GSH-Px ）, glutathione reductase （ GR ） and tumor necrosis factor- ct （ TNF- ct ）. Results ： Compared with that of mode group, the contents of ALT, AST and ALP decreased 31.3%, 35.2% and 50.7% （ P〈0.05 ）, respectively. The levels of SOD and GSH-Px in 20E treatment group increased 84.5% （ P〈0.01 ） and 105.5% （P〈0.01）than that in mode group, respectively. But, the ACT and TNF-o~ mRNA levels reduced 53.7% （P〈0.05）and 71.2% （P〈0.05）. Conclusion： 20E administration results in protection for acute liver injury of mice may be through enhancing liver anti-oxidative ability