黄连乌梅药对不同配伍比例的指纹图谱研究

Studies on Fingerprint of Coptis -Prunus Mum Couplet Medicines of Different Proportions

目的:通过对比黄连乌梅药对的HPLC指纹图谱,探讨该药对的配伍原理。方法:以色谱柱:AgilentHC-C18柱(4.6mm×250mm,5μm);检测波长:230nm;柱温:25℃为色谱条件,流动相:乙腈(A)-0.02mol/L磷酸二氢钾0.2%磷酸(B)梯度洗脱;检测波长:230nm;柱温:25℃为色谱条件,建立黄连乌梅药对的指纹图谱。结果:以黄连、乌梅单煎液作为对照,确定了13个共有峰,建立了不同配伍比例的指纹图谱。结论:乌梅与黄连的配伍比例趋近于1时,黄连中的盐酸药根碱、巴马汀、小檗碱溶出率达到最大。

Objective： Through the comparison of HPLC fingerprint of Coptis -Prunus mum couplet medicines, discusses the compatibility of the couplet. Methods ： The chromatographic fingerprints were obtained of the Coptis-Prunus mum couplet medicine on an Agilent HC-C18column （4.6mm × 250mm, 5 μm ） with the gradient elution solvent system of acetonitrile-0.02mol/L monopotassium phosphate 0.2% phosphoric. The column temperature was maintained at 25 ℃ and the detection wavelength was set at 230nm. Results ： Thirteen co-possessing peaks were selected as the fingerprint peaks and preliminary assignment was also performed by taking Coptis and Prunus mum prescription peak as the reference peak. Conclusion ： In the combination of Rhizoma coptidis and Prunus mum, the dissolution of berberine hydrochloride, plamatine hydrochloride and Jatrorrhizine Hydrochloride is maximum when the dosage percentage of them is 1：1.