径向流耦合超滤分离纯化铜藻多糖及其烟丝保润的应用

Separation of Sargassum Horneris Polysaccharides by Radial Flow Chromatography Coupling Ultrafiltration and Its Application in Tobacco Moisture Retention

研究铜藻提取液通过径向流色谱快速脱除蛋白质的工艺参数,以蛋白质脱出率和多糖保留率为考查指标,考察进样浓度、进样量、进样速度以及洗脱速度参数的影响。结果表明:当上样流速5 mL/min,洗脱速度40mL/min,上样量100 mL,上样浓度5mg/mL时,蛋白质脱除率为74.35%,多糖保留率为80.75%。利用超滤技术将通过径向流后和未通过径向流的铜藻多糖分离为10 ku以下、10～100 ku和100 ku以上各3个组分,通过对比多糖组分的蛋白质脱除率,得出径向流耦合超滤较单一法蛋白质脱除率上都有提高,耦合法脱除率达95%以上。对铜藻多糖各组分的保润性能进行测定,结果显示,铜藻多糖无论在防止解吸还是防止吸湿方面都有提高。

The process parameters of deproteinized of Sargassum Horneris extraction through radial flow chroma- tography was studied. Sample concentration, sample volume, sample flow-rate and elution flow-rate were studied on the influence of the deproteinization rate and polysaccharide recovery rate. The result showed that in the condition of sample flow-rate 5mL/min, the elution flow-rate 40mL/min, the sample volume 100mL and the sample concentration 5mg/mL, the deproteinization rate was 74.35% and the polysaccharide recovery rate was 80.75%. Furthermore, ul- trafiltration was applied to separate the polysaccharides of passed and residues of the radial flow chromatography into three groups： less than 10ku, between 10ku and 100ku and above 100ku. The comparison of protein removal rates from polysaccharides indicated that ultrafiltration coupled radial flow chromatography improved the protein removal rates to 96%. The moisture retention capacity of polysaccharides of Sargassum Horneri indicated that the ability of preventing desorption and moisture was both improved.