白桃成熟胚试管苗培养研究

A Study on Culture of the Test-tude Plantlets from Mature Embryo of White Peach

以白桃成熟胚为试验材料,无菌萌发获取试管苗,并筛选出试管苗培养最合适的增殖及生根培养基。研究结果表明,白桃试管苗培养最适合的增殖培养基为MS+6-BA 1.0 mg/L+IBA 0.0 mg/L+蔗糖30 g/L+琼脂6 g/L,增殖系数为3.45。生根率最佳诱导培养基为1/4 MS+IBA 0.0 mg/L+NAA 0.4 mg/L+蔗糖30 g/L+琼脂6 g/L,生根率为82.22%。生根数最佳诱导培养基为1/2 MS+IBA 0.5 mg/L+NAA 0.0 mg/L+蔗糖30 g/L+琼脂6 g/L,生根数为5.96。

The test-tube plantlets from mature embryos were used to screen the best proliferation medium and rooting medium. The results showed that the best proliferation medium was MS ＋ 6-BA 1.0 mg/L ＋ IBA 0.0 mg/L ＋ sucrose 30 g/L ＋ agar 6 g/L, with the proliferation coefficient of 3.45 ; the best medium for rooting rate was 1/4 MS ＋ IBA 0.0 mg/L ＋ NAA 0.4 mg/L ＋ sucrose 30 g/L ＋ agar 6 g/L, with the rooting rate of 82.22% ;the best medium for rooting number was 1/2 MS ＋ IBA 0.5 mg/L ＋ NAA 0.0 mg/L ＋ sucrose 30 g/L ＋ agar 6 g/L, with the average root number of 5.96 per plantlet.