Ca^(2+)/CaM信使对毛竹抗旱生理的影响

Effect of Ca^(2+)/CaM Messenger on Drought Resistance of Phyllostachys edulis

为了探讨钙信号对毛竹抗旱生理指标的影响,在水分胁迫下用EGTA、LaCl3、heparin、CPZ和CaCl2处理毛竹幼苗后,研究干旱胁迫下毛竹叶片内保护酶活性、脯氨酸、丙二醛、过氧化氢和可溶性蛋白的变化。结果表明:与对照相比,CaCl2处理明显提高了毛竹叶片保护酶活性和可溶性蛋白含量,降低了MDA、脯氨酸和过氧化氢含量;EGTA、LaCl3、heparin和CPZ处理提高了毛竹过氧化氢含量,抑制了SOD和CAT活性的上升,破坏了脯氨酸和可溶性蛋白的积累。但LaCl3和heparin处理对MDA含量的影响不大,heparin和CPZ处理对POD活性没有显著影响。这些结果暗示Ca2+/CaM信使可能主要通过调节抗氧化酶活性、脯氨酸和可溶性蛋白提高毛竹对干旱的适应性,且通过不同的传递路径调节以上生理指标。

The effect of calcium messenger on main drought resistance physiological indexes of Phyllostachys edulis was studied by investigating the activities of antioxidant enzymes, the content of proline, MDA, hydrogen peroxide and soluble protein in Phyllostachys edulis under water stress, after being blocked calcium messenger transduction by EGTA, LaCl3 and heparin, CPZ and CaCl2. Compared with the control, CaCl2 treatment increased SOD, POD and CAT enzyme activity, and soluble protein content, decreased MDA, proline and hydrogen peroxide content. Compared with the control, EGTA, LaCl3 and heparin, and CPZ could increase the hydrogen peroxide content, decrease SOD and CAT enzyme activity, and proline and soluble protein content. However, LaCl3 and heparin led to little changes of MDA content;heparin and CPZ had no obviously effect on POD enzyme activity. The results suggested that Ca2＋/CaM messenger might control drought resistance of Phyl- lostachys edulis by affecting activities of antioxidant enzymes and contents of proline and soluble protein through different transduction paths to affect them.