红麻花甲醇可溶性色素TLC分离与成分鉴定

A Study on the Separation by TLC and Initial Identification of Soluble Pigments from Kenaf Flower

以红麻花为原料,通过对红麻花甲醇可溶性色素的薄板层析(TLC)分离研究,得到其TLC最佳展层剂是:V(乙酸乙酯)∶V(水)∶V(甲酸)∶V(乙酸)=70∶30∶3∶2。以最佳展层剂展开花中的色素,得到4条颜色深浅不一的色带,反映该花色素至少含4种色素单体成分。对色素母液及第一单体成分样液进行290～700 nm扫描测定表明,色素母液在253.9 nm及372.6 nm各有一特征吸收峰;色素第一单体样液的特征吸收峰在252.7 nm及369.0 nm。初步鉴定结果揭示:红麻花甲醇可溶性色素成分主要为黄酮醇类。

Separating the methanol-soluble pigments from Kenaf flower by silca-G＆H thin-layer chromatography（ TLC ）, the optimum volume ratio of the separating layer reagent was obtained： ethyl acetate 70 to water 30 to methane acid 3 to acetic 2. Developing the flower pigment by the optimum layer reagent, four colour bands, were observed which indicated that the pigment had four monosomic pigment integrants. Sepemting and scanning results showed that the characteristic wave lengths of the pigment were 253.9 nm and 372.6 nm, the absorption peak of the first band was at 252.7 nm and 369.0 nm. Initial identification showed that the main methnol-soluble pigment component in kenal flower maybe belong to flavonols.