基于三维荧光光谱技术的牛奶中金霉素残留的检测研究

Detection of Chlortetracycline Residues in Milk Based on Three-dimensional Fluorescence Spectroscopy

牛奶中残留的抗生素易在人体内蓄积,进而对人体的健康产生隐患。研究表明,阳离子表面活化剂CT-MAB对金霉素(CTC)具有荧光增敏作用。采用一种新型的三维荧光光谱技术,对经过荧光增强后的含金霉素牛奶样品的三维荧光数据,应用三维-偏最小二乘法(3-PLS)进行其处理。结果表明:当CTC浓度在0.2～5 mg/L时,其校正组的相关系数R=0.989 0,校正样本的均方根误差RMSEC=0.219 9,预测组的相关系数R=0.985 5,均方根误差RMSEP=0.247 9。可见使用三维荧光光谱技术对牛奶中金霉素的残留进行快速检测是可行的。

Antibiotic residues in milk are inclined accumulate in human body, resulting a potential danger to health. According to research, CTMAB can enhance the fluorescence sensitivity of CTC. This study used a new type of three-dimensional fluorescence spectroscopy. Using the 3-PLS, the three-dimensional fluorescence data collected from the milk containing CTC were treated. The results showed that, when the concentration of CTC was 0.2-5 mg/L,the correlation coefficient in calibration set was O. 989 0 ,RMSEC =0.219 9 ,the correlation coefficient in prediction set was 0. 985 5 ,RMSEP =0. 247 9. It shows that it is feasible to use three-dimensional fluorescence spectroscopy for rapid detection of CTC in milk is feasible.